Capillary electrophoretic study of individual exocytotic events in single mast cells

In this work we have demonstrated the application of on-column dynamic release of serotonin from individual granules within rat peritoneal mast cells (RPMCs). These granules are approximately 0.25 fl in volume and represent some of the smallest entities studied by capillary electrophoresis (CE). Wit...

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Bibliographic Details
Published in:Journal of Chromatography A 1998-08, Vol.817 (1), p.377-382
Main Authors: Ho, Andrea M., Yeung, Edward S.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Electrophoresis, Capillary - methods
Exocytosis
Fundamental and applied biological sciences. Psychology
Humans
Male
Mast Cells - metabolism
Non peptidic neurotransmitters, polyamines
Other biological molecules
Peritoneal Cavity - cytology
Rats
Rats, Sprague-Dawley
Serotonin
Serotonin - metabolism
Spectrometry, Fluorescence
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Summary:In this work we have demonstrated the application of on-column dynamic release of serotonin from individual granules within rat peritoneal mast cells (RPMCs). These granules are approximately 0.25 fl in volume and represent some of the smallest entities studied by capillary electrophoresis (CE). With the coupling of high-speed CE and laser-induced native fluorescence, a time resolution of 0.002 min and a detection limit of 0.9 amol ( S/N=3, rms) were achieved. By using a secretagogue as the running buffer for CE, we resolved the peak profile of individual degranulation events released due to exocytosis. The average amount of serotonin released for five RPMCs analyzed was 33 amol ±16%.
ISSN:0021-9673
DOI:10.1016/S0021-9673(98)00551-2