Crystallization and preliminary X-ray studies of allophycocyanin from red alga Porphyra yezoensis

Allophycocyanin from red alga Porphyra yezoensis has been crystallized in three crystal forms. Form 1 crystals with space group P4132 or P4332 and cell parameters a = 286 Å, α = 90° were obtained by using isopropanol as precipitant. These crystals did not diffract beyond 5.8 Å and could not be used...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 1998-07, Vol.54 (4), p.662-664
Main Authors: Liu, Jin-yu, Zhang, Ji-ping, Wan, Zhu-li, Liang, Dong-cai, Zhang, Jian-ping, Wu, Hua-ju
Format: Article
Language:English
Subjects:
Crystallization
Crystallography, X-Ray
Phycocyanin - chemistry
Phycocyanin - isolation & purification
Plant Proteins - chemistry
Plant Proteins - isolation & purification
Protein Conformation
Rhodophyta - chemistry
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Summary:Allophycocyanin from red alga Porphyra yezoensis has been crystallized in three crystal forms. Form 1 crystals with space group P4132 or P4332 and cell parameters a = 286 Å, α = 90° were obtained by using isopropanol as precipitant. These crystals did not diffract beyond 5.8 Å and could not be used in structure analysis. Form 2 crystals were obtained when crystallization conditions were slightly changed by adding 0.2 M magnesium chloride. The space group of form 2 was not determined because it was difficult to get large single crystals. Form 3 crystals were obtained by using ammonium sulfate as precipitant. The space group of form 3 is R32 with cell dimensions a = b = 105.3, c = 189.4 Å and one unit in the asymmetric unit. These crystals diffract up to 2.06 Å resolution and are suitable for structure determination by molecular‐replacement methods.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444997017824