Characterization of the gene for human EMMPRIN, a tumor cell surface inducer of matrix metalloproteinases

• Published in: Gene 1998-10, Vol.220 (1), p.99-108
• Main Authors: Guo, Huiming, Majmudar, Gopa, Jensen, Timothy C, Biswas, Chitra, Toole, Bryan P, Gordon, Marion K
• Format: Article
• Language: English
• Subjects: 5' Untranslated Regions; Amino Acid Sequence; Animals; Antigens, CD; Antigens, Neoplasm; Base Sequence; Basigin; Biomarkers, Tumor - chemistry; Biomarkers, Tumor - genetics; CD147; Cloning, Molecular; Codon, Initiator - isolation & purification; Enzyme Induction; Exons; Humans; Introns; Membrane Glycoproteins - chemistry; Membrane Glycoproteins - genetics; Metalloendopeptidases - biosynthesis; Mice; Molecular Sequence Data; Transcription, Genetic; Tumor cell-derived collagenase stimulatory factor
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/S0378-1119(98)00400-4

EMMPRIN (extracellular matrix metalloproteinase inducer) also known as CD147 and basigin, is a member of the immunoglobulin family that is present on the surface of tumor cells and stimulates nearby fibroblasts to synthesize matrix metalloproteinases. Using our EMMPRIN cDNA, we have isolated a cosmid clone that contains the human EMMPRIN gene. S1 analysis with a fragment of the gene clone and primer extension of the mRNA was performed to determine the transcription start site. PCR and sequence analysis have defined the exon/intron organization of the gene and show that it is highly conserved with the mouse EMMPRIN/basigin gene. About 950 bases of the 5′-flanking region were examined for transcription factor consensus binding sites, locating three SP1 sites and two AP2 sites. The transcription start site was found to be located in a CpG island. Elements in the proximal promoter region were conserved in the human and mouse genes.