Presence of Dioxins in Human Follicular Fluid: Their Possible Stage-Specific Action on the Development of Preimplantation Mouse Embryos

Examination of human follicular fluid revealed the presence of polychlorinated dibenzodioxins (PCDDs) and dibenzofurans (PCDFs) at concentrations of approximately 1 pg/ml (0.01 pg TEQ/ml). To study their possible action, two-cell mouse embryos were cultured in the presence of 2,3,7,8-tetrachlorodibe...

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Published in:Biochemical and biophysical research communications 1998-09, Vol.250 (2), p.498-501
Main Authors: Tsutsumi, Osamu, Uechi, Hiroto, Sone, Hideko, Yonemoto, Junzo, Takai, Yasushi, Momoeda, Mikio, Tohyama, Chiharu, Hashimoto, Shunji, Morita, Masatoshi, Taketani, Yuji
Format: Article
Language:English
Subjects:
Animals
Blastocyst - drug effects
Dioxins - metabolism
Dioxins - pharmacology
Female
Follicular Fluid - metabolism
Humans
Mice
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Summary:Examination of human follicular fluid revealed the presence of polychlorinated dibenzodioxins (PCDDs) and dibenzofurans (PCDFs) at concentrations of approximately 1 pg/ml (0.01 pg TEQ/ml). To study their possible action, two-cell mouse embryos were cultured in the presence of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at concentrations between 0.5 and 100 pM and evaluated at 24-h intervals for their development to the eight-cell and blastocyst stages. The percentage of eight-cell embryos exposed to TCDD at 1, 2, and 5 pM concentrations was significantly lower than that of controls. However, blastocyst formation of the surviving eight-cell embryos was accelerated, with the number of cells in the blastocysts increased in a dose-dependent manner. Findings suggest that PCDDs and PCDFs may be present in human reproductive fluid and may exert some stage-specific effects on early embryonic development.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1998.9340