On the role of the general transcription factor Sp1 in the activation and repression of diverse mammalian oxidative phosphorylation genes

To gain insight into the role of the general transcription factor, Sp1, in the expression of nuclear genes involved in mitochondrial biogenesis, we investigated Sp1 activation of the adenine nucleotide translocator 2, cytochrome c1, F1-ATPase beta subunit, and the mitochondria transcription factor (...

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Bibliographic Details
Published in:Journal of bioenergetics and biomembranes 1999-04, Vol.31 (2), p.129-135
Main Authors: Zaid, A, Li, R, Luciakova, K, Barath, P, Nery, S, Nelson, B D
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cell Line
Cellular biology
Cytochromes c1 - genetics
DNA-Binding Proteins - genetics
Drosophila melanogaster
Gene Expression Regulation
Humans
Kinetics
Mammals
Mitochondria - genetics
Mitochondria - metabolism
Mitochondrial ADP, ATP Translocases - genetics
Mitochondrial Proteins
Molecular Sequence Data
Nuclear Proteins
Oxidative Phosphorylation
Promoter Regions, Genetic
Proteins
Proton-Translocating ATPases - genetics
Recombinant Proteins - metabolism
Sp1 Transcription Factor - genetics
Sp1 Transcription Factor - metabolism
Suppression, Genetic
Transcription Factors - genetics
Transfection
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Summary:To gain insight into the role of the general transcription factor, Sp1, in the expression of nuclear genes involved in mitochondrial biogenesis, we investigated Sp1 activation of the adenine nucleotide translocator 2, cytochrome c1, F1-ATPase beta subunit, and the mitochondria transcription factor (mtTFA) promoters transfected into Drosophila cell lines. The numbers and organization of GC elements vary in the four promoters, but the magnitude of activation by coexpressed human Sp1 was similar. A feature common to the four promoters is the presence of multiple, proximal Sp1-activating elements that account for 50% or more of the transcription activation by Sp1. The distribution and function of individual distal Sp1 elements is less defined and appear to be more promoter-specific. Finally, data from transfected Drosophila cells provide the first direct proof for the involvement of Sp1 in the negative regulation of the ANT2 promoter and as a possible participant in repression of the beta-subunit promoter. The role of Sp1 in both the positive and negative regulation of OXPHOS promoters is unique.
ISSN:0145-479X
1573-6881
DOI:10.1023/A:1005499727732