A Novel Histone Acetyltransferase Is an Integral Subunit of Elongating RNA Polymerase II Holoenzyme

The elongator complex is a major component of the RNA polymerase II (RNAPII) holoenzyme responsible for transcriptional elongation in yeast. Here we identify Elp3, the 60-kilodalton subunit of elongator/RNAPII holoenzyme, as a highly conserved histone acetyltransferase (HAT) capable of acetylating c...

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Published in:Molecular cell 1999-07, Vol.4 (1), p.123-128
Main Authors: Wittschieben, Birgitte Ø, Otero, Gabriel, de Bizemont, Therese, Fellows, Jane, Erdjument-Bromage, Hediye, Ohba, Reiko, Li, Yang, Allis, C.David, Tempst, Paul, Svejstrup, Jesper Q
Format: Article
Language:English
Subjects:
Acetyltransferases - chemistry
Acetyltransferases - genetics
Amino Acid Sequence
Chromatin - chemistry
Cloning, Molecular
Fungal Proteins - chemistry
Fungal Proteins - genetics
Gene Deletion
Histone Acetyltransferases
Molecular Sequence Data
Phenotype
Recombinant Proteins - genetics
RNA Polymerase II - chemistry
RNA Polymerase II - metabolism
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Sequence Alignment
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Summary:The elongator complex is a major component of the RNA polymerase II (RNAPII) holoenzyme responsible for transcriptional elongation in yeast. Here we identify Elp3, the 60-kilodalton subunit of elongator/RNAPII holoenzyme, as a highly conserved histone acetyltransferase (HAT) capable of acetylating core histones in vitro. In vivo, ELP3 gene deletion confers typical elp phenotypes such as slow growth adaptation, slow gene activation, and temperature sensitivity. These results suggest a role for a novel, tightly RNAPII-associated HAT in transcription of DNA packaged in chromatin.
ISSN:1097-2765
1097-4164
1097-4164
DOI:10.1016/S1097-2765(00)80194-X