Double-stranded RNA in the entomopathogenic fungus Metarhizium flavoviride

Bands of dsRNA were detected in five out of seven isolates of the entomopathogenic fungus Metarhizium flavoviride. The identity of these bands was proven by RNase and S1-nuclease treatments. The transference of dsRNA between isolates (from CG291 to CG442) was successfully carried out through forced...

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Bibliographic Details
Published in:Current genetics 1999-08, Vol.36 (1/2), p.94-97
Main Authors: Martins, M.K, Furlaneto, M.C, Sosa-Gomez, D.R, Faria, M.R, Fungaro, M.H.P
Format: Article
Language:English
Subjects:
Acrididae
Animals
Ascomycota - cytology
Ascomycota - genetics
Ascomycota - pathogenicity
Cell Nucleus - genetics
experimental infections
Genotype
Grasshoppers - microbiology
Metarhizium flavoviride
mutants
Mutation - genetics
Polymorphism, Genetic - genetics
Rhammatocerus schistocercoides
Ribonucleases - metabolism
RNA
RNA, Double-Stranded - genetics
RNA, Double-Stranded - metabolism
RNA, Fungal - genetics
RNA, Fungal - metabolism
Single-Strand Specific DNA and RNA Endonucleases - metabolism
virulence
Virulence - genetics
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Summary:Bands of dsRNA were detected in five out of seven isolates of the entomopathogenic fungus Metarhizium flavoviride. The identity of these bands was proven by RNase and S1-nuclease treatments. The transference of dsRNA between isolates (from CG291 to CG442) was successfully carried out through forced heterokaryons. Isogenic strains, with or without dsRNA, were submitted to virulence tests against the grasshopper Rhammatocerus schistocercoides. In contrast to what has been found in some phytopathogenic fungi, these dsRNA fragments did not cause hypovirulence to M. flavoviride.
ISSN:0172-8083
1432-0983
DOI:10.1007/s002940050477