Differential regulation of somatostatin receptor type 2 (sst 2) expression in AR4-2J tumor cells implanted into mice during octreotide treatment

• Published in: Cancer research (Chicago, Ill.) Ill.), 1999-08, Vol.59 (15), p.3652-3657
• Main Authors: FROIDEVAUX, S, HINTERMANN, E, TÖRÖK, M, MÄCKE, H. R, BEGLINGER, C, EBERLE, A. N
• Format: Article
• Language: English
• Subjects: Animals; Antineoplastic agents; Antineoplastic Agents, Hormonal - pharmacology; Antineoplastic Agents, Hormonal - therapeutic use; Biological and medical sciences; Chemotherapy; Cycloheximide - pharmacology; Dactinomycin - pharmacology; Gene Expression Regulation, Neoplastic - drug effects; Medical sciences; Mice; Mice, SCID; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - drug effects; Neoplasm Proteins - genetics; Nucleic Acid Synthesis Inhibitors - pharmacology; Octreotide - pharmacology; Octreotide - therapeutic use; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Pharmacology. Drug treatments; Protein Synthesis Inhibitors - pharmacology; Rats; Receptors, Somatostatin - biosynthesis; Receptors, Somatostatin - drug effects; Receptors, Somatostatin - genetics; Somatostatin - physiology; Tumor Cells, Cultured; Up-Regulation - drug effects
• ISSN: 0008-5472; 1538-7445

Octreotide is a somatostatin analogue that is widely used for cancer therapy and tumor imaging. Its efficacy in tumors depends mainly on the expression of the somatostatin receptor type 2 (sst 2). Desensitization and down-regulation of sst 2 after agonist exposure can have important consequences for patients under ongoing octreotide therapy because it may induce temporary tumor unresponsiveness and impair sst 2-based tumor scintigraphy. Therefore, we have investigated the effect of octreotide on sst 2 expression in vitro, as well as in a tumor mouse model. In vitro, short exposure to octreotide induced rapid dose-dependent down-regulation of sst 2 in the rat pancreatic AR4-2J cell line. Within 0.5 h, 80% of sst 2 had disappeared from the cell surface. A total recovery required 24 h and was shown to depend on protein synthesis, but not on new sst 2 mRNA transcription, indicating that sst 2 was probably degraded during the down-regulation process. Similar results were obtained in vivo. On the other hand, long-term continuous release of octreotide for 7 days, as achieved with octreotide-containing osmotic minipumps, caused sst 2 up-regulation in vivo, but not in vitro. Furthermore, this up-regulation of sst 2 in tumor-bearing scid mice was shown to depend on constant exposure of the animals to octreotide, as it was not observed when octreotide was given discontinuously in two s.c. daily injections. These results demonstrate that the continuous release of a small amount of octreotide, which in cancer therapy may be achieved with long-acting release formulations of the peptide, can induce sst 2 up-regulation on cancer cells. This may improve the efficacy of both tumor imaging and long-term octreotide therapy.