Expression of Functional Tissue Factor on Small Vesicles of Lipopolysaccharide- Stimulated Human Vascular Endothelial Cells

We examined tissue factor expression on lipopolysaccharide-stimulated endothelial cells and their small vesicles by using specific antibodies and flow cytometry. Tissue factor functional activity was also assessed by activation of factor X. Endothelial cells were stimulated with 10 μg/ml of lipopoly...

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Bibliographic Details
Published in:Thrombosis research 1998-09, Vol.91 (6), p.297-304
Main Authors: Kagawa, Hideo, Komiyama, Yutaka, Nakamura, Shin, Miyake, Tetsuya, Miyazaki, Yasuhiko, Hamamoto, Kenjirou, Masuda, Midori, Takahashi, Hakuo, Nomura, Shosaku, Fukuhara, Shirou
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blood coagulation. Blood cells
Cells, Cultured
Disseminated Intravascular Coagulation - physiopathology
Endothelial cells
Endothelium, Vascular - drug effects
Endothelium, Vascular - ultrastructure
Enzyme Activation
Factor X - metabolism
Factor Xa - biosynthesis
Flow cytometry
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation
General aspects, investigation methods, hemostasis, fibrinolysis
Humans
Lipopolysaccharides - pharmacology
Microscopy, Immunoelectron
Molecular and cellular biology
Small vesicles
Thromboplastin - biosynthesis
Thromboplastin - genetics
Tissue factor
Umbilical Veins
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Summary:We examined tissue factor expression on lipopolysaccharide-stimulated endothelial cells and their small vesicles by using specific antibodies and flow cytometry. Tissue factor functional activity was also assessed by activation of factor X. Endothelial cells were stimulated with 10 μg/ml of lipopolysaccharide in M-199/bovine serum albumin. Flow cytometry showed that expression of tissue factor on endothelial cells reached a maximum at 6 hours after stimulation, whereas that on small vesicles reached a maximum after 12 hours. Factor X activation mediated by factor VIIa and tissue factor was observed over a similar time course and was inhibited by the addition of antitissue factor antibody. Immunoelectron microscopy suggested that small vesicles with expression of some tissue factor were produced from the surface of endothelial cells. Our findings thus showed that tissue factor on endothelial cells produced by lipopolysaccharide stimulation was partly released to small vesicles. This may cause disseminated intravascular coagulation and related coagulation disorders.
ISSN:0049-3848
1879-2472
DOI:10.1016/S0049-3848(98)00108-X