Juvenile female Litomosoides sigmodontis produce an excretory/secretory antigen (Juv-p120) highly modified with dimethylaminoethanol

A 120 kDa antigen produced by juvenile female Litomosoides sigmodontis (Juv-p120) was isolated and purified. The amino acid composition of the molecule was determined. Juv-p120 was shown to be highly modified with N,N-dimethyl-aminoethanol (28·4 mol%). Treatment of Juv-p120 with potassium hydroxide...

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Bibliographic Details
Published in:Parasitology 1998-09, Vol.117 (3), p.265-271, Article S0031182098002996
Main Authors: HINTZ, M., SCHARES, G., TAUBERT, A., GEYER, R., ZAHNER, H., STIRM, S., CONRATHS, F. J.
Format: Article
Language:English
Subjects:
Amidohydrolases - chemistry
Amino Acids - analysis
Animals
Antibodies, Helminth - biosynthesis
Antigens, Helminth - chemistry
Antigens, Helminth - isolation & purification
Antigens, Helminth - metabolism
Biological and medical sciences
Blotting, Western
Carbohydrates - analysis
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Deanol - chemistry
dimethylaminoethanol
Electrophoresis, Polyacrylamide Gel
Endopeptidase K - chemistry
excretory/secretory products
Female
filariasis
Filariasis - immunology
Filarioidea - immunology
Fundamental and applied biological sciences. Psychology
Glycoside Hydrolases - chemistry
Hexosaminidases - chemistry
Host parasite relation
pathogenicity
Hydroxides - chemistry
Invertebrates
Litomosoides sigmodontis
Muridae
Nemathelminthia. Plathelmintha
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Periodic Acid - chemistry
Potassium Compounds - chemistry
Protein Processing, Post-Translational
Rabbits
Sigmodontinae
Type C Phospholipases - chemistry
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Summary:A 120 kDa antigen produced by juvenile female Litomosoides sigmodontis (Juv-p120) was isolated and purified. The amino acid composition of the molecule was determined. Juv-p120 was shown to be highly modified with N,N-dimethyl-aminoethanol (28·4 mol%). Treatment of Juv-p120 with potassium hydroxide (β-elimination) or with sodium m-periodate leads to the destruction of epitopes recognized by antibodies immune affinity-purified with isolated Juv-p120. Juvenile L. sigmodontis were shown to release Juv-p120 into the pleural cavity of infected Mastomys coucha before the onset of patency.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182098002996