Cautionary note on the use of end-labelling DNA fragments for detection of apoptosis

The gastrointestinal epithelum undergoes a continual process of cell renewal which is partly regulated by apoptosis. The process of growth and differentiation is greatly enhanced in the terminal ileum after massive small bowel resection, a well-established model of intestinal adaptation. We have app...

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Bibliographic Details
Published in:Pathology 1998-08, Vol.30 (3), p.267-271
Main Authors: Wolvekamp, Monique C.J., Darby, Ian A., Fuller, Peter J.
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Apoptosis - genetics
Biological and medical sciences
cell death
Deoxyuracil Nucleotides
Dideoxynucleotides
Digoxigenin - analogs & derivatives
Disease Models, Animal
DNA - analysis
Female
Ileum - pathology
Ileum - surgery
Immunoenzyme Techniques
In Situ Nick-End Labeling - methods
Indicators and Reagents
intestine
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Miscellaneous. Technology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Rats
Rats, Sprague-Dawley
Sensitivity and Specificity
skin
Skin - injuries
Tropical medicine
TUNEL
wound
Wound Healing - physiology
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Summary:The gastrointestinal epithelum undergoes a continual process of cell renewal which is partly regulated by apoptosis. The process of growth and differentiation is greatly enhanced in the terminal ileum after massive small bowel resection, a well-established model of intestinal adaptation. We have applied the terminal deoxynucleotidyl transferase (TdT)-mediated UTP nick end-labelling (TUNEL) method to establish the role of apoptosis in intestinal adaption in terminal ileum of control animals after small bowel resection. Healing skin wound and lymph node were used as positive control tissue for apoptotic cells. We report that considerable inter-animal variation was observed in the intestinal tissue. We believe that caution is required in the interpretation of TUNEL staining in intestinal tissue and in its use as a specific marker for apoptosis in this setting.
ISSN:0031-3025
1465-3931
DOI:10.1080/00313029800169426