Juvenile hormone inhibition of gene expression for cytochrome P4504C1 in adult females of the cockroach, Blaberus discoidalis

The regulation and pattern of gene expression for cytochrome P4504C1 was measured in the fat body of adult females of the cockroach, Blaberus discoidalis. The level of CYP4C1-mRNA was high at adult emergence but disappeared after 4 days of adult life. In starved females, CYP4C1-mRNA levels declined...

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Bibliographic Details
Published in:Insect biochemistry and molecular biology 1999-08, Vol.29 (8), p.667-673
Main Authors: Lu, Kuang-Hui, Bradfield, James Y., Keeley, Larry L.
Format: Article
Language:English
Subjects:
age
Age Factors
allatectomy
Animals
Blaberus discoidalis
Cockroach
Cockroaches - enzymology
corpora allata
CYP4C1 gene
cytochrome P-450
Cytochrome P-450 Enzyme System - genetics
Cytochrome P450
decapitation
fat body
Feeding Behavior
Female
gender differences
Gene expression
Gene Expression Regulation, Enzymologic
hormonal regulation
Hypertrehalosemic hormoen
hypertrehalosemic hormone
inhibition
Insect Hormones
Insects
juvenile hormone
juvenile hormones
Juvenile Hormones - physiology
Male
messenger RNA
Methoprene
neurochromes
neurohormones
Neuropeptides
RNA, Messenger
Sexual Behavior, Animal
starvation
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Summary:The regulation and pattern of gene expression for cytochrome P4504C1 was measured in the fat body of adult females of the cockroach, Blaberus discoidalis. The level of CYP4C1-mRNA was high at adult emergence but disappeared after 4 days of adult life. In starved females, CYP4C1-mRNA levels declined by day 4 but increased steadily thereafter; by 25 days, the levels were nearly twice those observed at eclosion. Both the rapid early disappearance of the transcript and the starvation-related increase failed to occur following decapitation. Allatectomy also prevented the disappearance of CYP4C1-mRNA at day 4, and treatment of decapitated females with methoprene (JHA) stimulated a 70% decrease in transcript within 24 h. Injection of synthetic Blaberus hypertrehalosemic hormone (HTH) increased CYP4C1-mRNA by six-fold in the fat body of both intact and decapitated females. CYP4C1-mRNA in the fat body of males did not respond to JHA treatment. The dynamics of CYP4C1-mRNA in the fat body of females could be explained based on an inhibition of CYP4C1 expression by JH that was overcome by HTH.
ISSN:0965-1748
1879-0240
DOI:10.1016/S0965-1748(99)00034-X