Detection of a chemotactic factor for neutrophils in extracts of female Onchocerca volvulus

Neutrophilic granulocytes and macrophages are the dominant inflammatory cell types observed in the vicinity of and attached to adult Onchocerca volvulus in the subcutaneous nodules. Crude extract from female O. volvulus was examined for chemotactic activity for peripheral neutrophils from healthy in...

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Bibliographic Details
Published in:Acta tropica 1998-08, Vol.71 (1), p.45-56
Main Authors: Rubio de Krömer, M.T, Krömer, M, Lüersen, K, Brattig, N.W
Format: Article
Language:English
Subjects:
Africa, Western
Animals
Biological and medical sciences
Chemotactic factor
Chemotactic Factors - analysis
Chemotaxis, Leukocyte - immunology
Chromatography, Gel
Diseases caused by nematodes
Female
Filariases
Glucuronidase - analysis
Helminthic diseases
Humans
Immunohistochemistry
Immunohistochemistry of onchocercoma
Infectious diseases
Male
Medical sciences
Neutrophils
Neutrophils - enzymology
Neutrophils - immunology
Neutrophils - pathology
Onchocerca volvulus
Onchocerca volvulus - cytology
Onchocerca volvulus - immunology
Onchocerciasis - immunology
Onchocerciasis - pathology
Onchocercosis
Parasitic diseases
Tropical medicine
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Summary:Neutrophilic granulocytes and macrophages are the dominant inflammatory cell types observed in the vicinity of and attached to adult Onchocerca volvulus in the subcutaneous nodules. Crude extract from female O. volvulus was examined for chemotactic activity for peripheral neutrophils from healthy individuals by use of an endogenous component chemotactic assay in Boyden chambers. Significant chemotactic responses of neutrophils were detected using O. volvulus extracts at ≥15 μg/ml in a dose-dependent manner. Checkerboard analysis demonstrated low chemokinetic in addition to chemotactic activity. Neutrophil migration was also elicited by excretory-secretory products of vital females. Fractionation of the female worm extract by FPLC revealed two components with chemotactic activity, one with a molecular mass less than 12 kDa and another with a molecular mass of >200 kDa. Immunohistological examination of onchocercomas containing only one adult alive filarial worm demonstrated that neutrophils were accumulated near and attached to the cuticle of immature females, females producing microfilariae and males.
ISSN:0001-706X
1873-6254
DOI:10.1016/S0001-706X(98)00044-8