Determination of Nitrite in Waters by Microplate Fluorescence Spectroscopy and HPLC with Fluorescence Detection

A selective and versatile fluorescence spectroscopic method for the determination of nitrite in waters has been developed. Nitrite reacts in the presence of mineral acids with the nonfluorescent N-methyl-4-hydrazino-7-nitrobenzofurazan forming N-methyl-4-amino-7-nitrobenzofurazan, which can be detec...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1999-08, Vol.71 (15), p.3003-3007
Main Authors: Büldt, Andrea, Karst, Uwe
Format: Article
Language:English
Subjects:
Analytical chemistry
Applied sciences
Chemicals
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - methods
Continental surface waters
Exact sciences and technology
Fluorescence
Hydrazines - chemistry
Molecular Structure
Natural water pollution
Nitrites - analysis
Other chromatographic methods
Oxadiazoles - chemistry
Pollution
Scientific imaging
Spectrometry, Fluorescence - instrumentation
Spectrometry, Fluorescence - methods
Ultraviolet Rays
Water
Water - analysis
Water treatment and pollution
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Summary:A selective and versatile fluorescence spectroscopic method for the determination of nitrite in waters has been developed. Nitrite reacts in the presence of mineral acids with the nonfluorescent N-methyl-4-hydrazino-7-nitrobenzofurazan forming N-methyl-4-amino-7-nitrobenzofurazan, which can be detected by fluorescence spectroscopy with an excitation maximum at λ = 468 nm and an emission maximum at λ = 537 nm in acetonitrile. Three new methods based on this reaction have been developed:  Direct fluorescence spectroscopy, HPLC/fluorescence, or HPLC with UV/vis detector may be selected as detection techniques. On microplates, high-throughput fluorescence spectroscopy is achieved, while HPLC/fluorescence provides lower limits of detection, and HPLC with UV/vis detection enables evaluation of the reaction with standard instrumentation. Different water samples were investigated using all detection modes, and a photometric standard procedure was successfully employed to validate the new methods with an independent technique.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac981330t