Comparison of genomic DNA in various strains of Boophilus microplus (Acari: Ixodidae)

The conventional method of identifying acaricide resistance in a suspect tick population by the United Nations Food and Agriculture Organization packet assay is a laborious and time-consuming process. DNA probes have been demonstrated as rapid and accurate tools for detecting pesticide resistance in...

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Bibliographic Details
Published in:Journal of medical entomology 1998-09, Vol.35 (5), p.895-900
Main Authors: Hernandez, R. (Texas AandM University, College Station, TX.), Chen, A.C, Davey, R.B, Ivie, G.W, Wagner, G, George, J.E
Format: Article
Language:English
Subjects:
ACARICIDAS
ACARICIDE
ACARICIDES
Animals
Biological and medical sciences
BOOPHILUS MICROPLUS
DNA Probes
Fundamental and applied biological sciences. Psychology
GENETIC VARIATION
Genome
Insecticide Resistance
Ixodidae
Larva
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
Nuisances
RANDOM AMPLIFIED POLYMORPHIC DNA
Random Amplified Polymorphic DNA Technique
RAPD
RESISTANCE AUX PRODUITS CHIMIQUES
RESISTANCE TO CHEMICALS
RESISTENCIA A PRODUCTOS QUIMICOS
Ticks - classification
Ticks - genetics
VARIACION GENETICA
VARIATION GENETIQUE
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Summary:The conventional method of identifying acaricide resistance in a suspect tick population by the United Nations Food and Agriculture Organization packet assay is a laborious and time-consuming process. DNA probes have been demonstrated as rapid and accurate tools for detecting pesticide resistance in insect species. Random-amplified polymorphic DNA (RAPD) has been used by other groups to differentiate species of mosquitoes and populations within a mosquito species. By using different arbitrary oligonucleotides as primers with RAPD, we have demonstrated that various strains of Boophilus microplus (Canestrini) show different patterns of DNA fragments on agarose gel electrophoresis. The unique DNA fragments may be useful for developing probes that can detect acaricide resistance in field pest populations
ISSN:0022-2585
1938-2928
DOI:10.1093/jmedent/35.5.895