Allele resolution of HLA-A using oligonucleotide probes in a two-stage typing strategy

High‐resolution polymerase chain reaction using sequence‐specific oligonucleotide probes (PCR‐SSOP) typing methods for HLA‐A identification have been established. The four systems, which operate independently of each other, are intended for use as secondary typing systems following HLA‐A identificat...

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Bibliographic Details
Published in:Tissue antigens 1999-07, Vol.54 (1), p.59-68
Main Authors: Williams, F., Meenagh, A., Maxwell, A.p., Middleton, D.
Format: Article
Language:English
Subjects:
Alleles
histocompatibility locus HLA
HLA-A Antigens - genetics
HLA-A high-resolution
Humans
Ireland
Oligonucleotide Probes
oligonucleotides
Polymerase Chain Reaction - methods
sequence-specific oligonucleotide probes
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Summary:High‐resolution polymerase chain reaction using sequence‐specific oligonucleotide probes (PCR‐SSOP) typing methods for HLA‐A identification have been established. The four systems, which operate independently of each other, are intended for use as secondary typing systems following HLA‐A identification with a medium‐resolution PCR‐SSOP technique. The systems, all using digoxigenin‐labelled probes, are based on group specific amplifications for resolution of: i) HLA‐A*29 & ‐A*33; ii) HLA‐A*24 & ‐A*30; and iii) HLA‐A*26, ‐A*25, ‐A*11, ‐A*34, ‐A*66 and ‐A*68 alleles, respectively. The fourth system, for the detection of HLA‐A*02 alleles, is a modification of a previously reported PCR‐SSOP subtyping system. The methods have been applied to individuals from the local bone marrow registry and HLA‐A allele frequencies for the Northern Ireland population have been established.
ISSN:0001-2815
1399-0039
DOI:10.1034/j.1399-0039.1999.540107.x