Crosslinking of enzymes for improved stability and performance

Stabilization of enzymes without diminishing and diluting their activity is crucial for their use as industrial catalysts. One practically proven approach involves crystallization of the enzyme (size 1–200 μm) followed by subsequent crosslinking. In many cases, the resulting crosslinked enzyme cryst...

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Bibliographic Details
Published in:Current opinion in biotechnology 1999-08, Vol.10 (4), p.331-335
Main Author: Govardhan, Chandrika P
Format: Article
Language:English
Subjects:
Biotechnology
Cross-Linking Reagents
Crystallization
Enzyme Stability
Enzymes - chemistry
Enzymes - metabolism
In Vitro Techniques
Protein Engineering
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Summary:Stabilization of enzymes without diminishing and diluting their activity is crucial for their use as industrial catalysts. One practically proven approach involves crystallization of the enzyme (size 1–200 μm) followed by subsequent crosslinking. In many cases, the resulting crosslinked enzyme crystals exhibit increased storage stability as well as stability in applications. The technology is complementary to protein engineering methods that aim at boosting the inherent stability of the enzyme.
ISSN:0958-1669
1879-0429
DOI:10.1016/S0958-1669(99)80060-3