Characterization of Acholeplasma laidlawii ftsZ Gene and Its Gene Product

The ftsZ gene was found among representatives of all bacterial groups. FtsZ protein is an essential component of cell division ring. Contraction of this cytoskeleton-like ring is believed to be the universal way of bacterial division. Acholeplasma laidlawii possesses all features of the minimal myco...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1999-08, Vol.262 (1), p.44-49
Main Authors: Kukekova, A.V, Malinin, A.Yu, Ayala, J.A, Borchsenius, S.N
Format: Article
Language:English
Subjects:
Acholeplasma
Acholeplasma laidlawii
Acholeplasma laidlawii - genetics
Acholeplasma laidlawii - immunology
Amino Acid Sequence
Antibodies, Bacterial - immunology
Antibody Specificity
Bacterial Proteins - biosynthesis
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - immunology
Base Sequence
Cloning, Molecular
Cytoskeletal Proteins
Escherichia coli - genetics
Evolution, Molecular
ftsZ gene
gene expression
Genes, Bacterial - genetics
Genetic Variation - genetics
Isoelectric Point
Molecular Sequence Data
Molecular Weight
Mycoplasma
Open Reading Frames - genetics
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - chemistry
Recombinant Fusion Proteins - immunology
Sequence Alignment
Sequence Homology, Amino Acid
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Summary:The ftsZ gene was found among representatives of all bacterial groups. FtsZ protein is an essential component of cell division ring. Contraction of this cytoskeleton-like ring is believed to be the universal way of bacterial division. Acholeplasma laidlawii possesses all features of the minimal mycoplasma cell and some traits of cell-wall bacteria and seems to be a promising object for study of basic principles of the bacterial division process. We cloned an A. laidlawii chromosomal fragment containing ftsZ gene and two flanking orf which also were identified. A. laidlawii FtsZ protein has been determined with polyclonal antibodies raised in rabbit. It was demonstrated that ftsZ gene of A. laidlawii could be expressed in E. coli cells. We also revealed that A. laidlawii FtsZ had a low similarity to proteins of Mycoplasma genitalium and M. pneumoniae. The comparison of FtsZ structures may be used for investigation of bacterial phylogenetic relations.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.1135