A rapid (20 h) solid screening medium for detecting methicillin-resistant Staphylococcus aureus

Methicillin aztreonam mannitol salt agar is a sensitive and reliable solid screening medium for detecting methicillin-resistant Staphylococcus aureus (MRSA). With this medium an incubation period of only 20 h is sufficient to either produce visible colonies of MRSA or to exclude MRSA (no staphylococ...

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Bibliographic Details
Published in:The Journal of hospital infection 1998-09, Vol.40 (1), p.67-72
Main Authors: Perry, P.L., Coombs, G.W., Boehm, J.D., Pearman, J.W.
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Carrier State - diagnosis
Carrier State - microbiology
Culture Media
Fundamental and applied biological sciences. Psychology
Humans
Methicillin - pharmacology
Methicillin Resistance
methicillin-resistant Staphylococcus aureus (MRSA)
Microbiology
selective screening medium
Staphylococcal Infections - prevention & control
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - growth & development
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Summary:Methicillin aztreonam mannitol salt agar is a sensitive and reliable solid screening medium for detecting methicillin-resistant Staphylococcus aureus (MRSA). With this medium an incubation period of only 20 h is sufficient to either produce visible colonies of MRSA or to exclude MRSA (no staphylococcal colonies). Coagulase testing (requiring a further 6 h) enables coagulase-positive isolates to be provisionally reported as ‘possible MRSA’ 26–30 h after the swabs were collected. The medium supports growth of intrinsically resistant staphylococci including low-expression-class MRSA (methicillin minimum inhibitory concentration (MIC) 8–16mg/L), but methicillin susceptible staphylococci and β-lactamase hyperproducers are suppressed.
ISSN:0195-6701
1532-2939
DOI:10.1016/S0195-6701(98)90027-5