Organization and expression of mouse nm23-M1 gene. Comparison with nm23-M2 expression

Nm23 is a gene family encoding different isoforms of the nucleotide diphosphate kinase (NDPK), an enzyme involved in the synthesis of nucleoside triphosphates. In the present study, the organization and expression of the nm23-M1 gene encoding the mouse NDPKA isoform are described. This gene is about...

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Bibliographic Details
Published in:Gene 1999-08, Vol.236 (2), p.221-230
Main Authors: Dabernat, Sandrine, Larou, Monique, Massé, Karine, Dobremez, Eric, Landry, Marc, Mathieu, Claudine, Daniel, Jean-Yves
Format: Article
Language:English
Subjects:
Animals
Chromosome Mapping
Exons
Genomic Library
In Situ Hybridization
Intestinal Mucosa - embryology
Mice
Mice, Inbred C57BL
Models, Genetic
Monomeric GTP-Binding Proteins
NDP kinase A and B
NM23 Nucleoside Diphosphate Kinases
Nucleoside-Diphosphate Kinase - genetics
Polymerase Chain Reaction
Promoter Regions, Genetic
Sequence Homology, Amino Acid
Thymus Gland - embryology
Tissue Distribution
Transcription
Transcription Factors - analysis
Transcription Factors - genetics
Transcription Factors - metabolism
Transcription, Genetic
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Summary:Nm23 is a gene family encoding different isoforms of the nucleotide diphosphate kinase (NDPK), an enzyme involved in the synthesis of nucleoside triphosphates. In the present study, the organization and expression of the nm23-M1 gene encoding the mouse NDPKA isoform are described. This gene is about 10 kb long and composed of five exons. The organization and the exon–intron boundaries are strictly conserved as compared to the human and rat related genes. The gene promoter region did not exhibit any consensus TATA box, SP1 binding element or Inr sequence. By contrast, TCF-1/LEF-1 binding elements and Pit-1 consensus sequence were present. Northern blotting and in situ hybridization methods were carried out in adult and 18.5 days post-coitum (dpc) mouse embryo, respectively. They showed tissue-specific expression of nm23-M1 transcripts, despite housekeeping gene promoter features. The strongest signals were detected in the nervous system, sensory organs and embryonic thymus. In contrast nm23-M2 mRNA was shown to be more widely expressed. The relationship between nm23-M1 gene tissue-specific expression and the putative binding element of the promoter region is discussed.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(99)00288-7