Prostanoid synthesis in whole blood cells from fish of the Arabian Gulf

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 1998-04, Vol.119 (4), p.639-646
• Main Authors: Thomson, Martha, Al-Hassan, Jassim M, Al-Saleh, Jinan, Fayad, Sanaa', Ali, Muslim
• Format: Article
• Language: English
• Subjects: 6-Ketoprostaglandin F1 alpha - biosynthesis; Animals; Arabian Gulf; Arachidonic Acid - metabolism; Arius; Blood cells; Blood Cells - enzymology; Blood Cells - metabolism; Carcharhinus; Catfishes - metabolism; Chiloscyllium griseum; Chromatography, Thin Layer; Fish; Fishes - metabolism; Marine; Prostaglandin-Endoperoxide Synthases - metabolism; Prostaglandins; Prostaglandins - biosynthesis; Prostaglandins - blood; Saudi Arabia; Sharks - metabolism; Thromboxane; Thromboxane B2 - biosynthesis; Thromboxanes - biosynthesis
• ISSN: 1096-4959; 0305-0491; 1879-1107
• DOI: 10.1016/S0305-0491(98)00039-X

The ability to synthesise prostaglandins and thromboxane from 14C-labelled arachidonic acid was investigated in 11 species of fish from the Arabian Gulf. Cyclooxygenase activity was assessed in washed whole blood cells. Arachidonic acid and its metabolites were extracted and separated on silicic acid columns and thin layer chromatography (silica gel G). Total capacity to convert [ 14C]arachidonic acid to prostanoids varied from 1 to 35% among the 11 fish species studied. Gray shark ( Chiloscyllium griseum) blood cells had the highest capacity (37±0.4%) to convert arachidonate into prostanoids and two species of catfish ( Arius bilineatus and A. thalassinus) exhibited greater than 10% capacity to convert [ 14C]arachidonate into prostanoids. The major prostanoid synthesised by the two catfish ( A. bilineatus and A thalassinus) was 6-keto PGF 1α, a stable metabolite of prostacyclin, PGI 2. In contrast, A. teunispinis synthesised thromboxane B 2, a stable metabolite of thromboxane A 2. Thromboxane B 2 (TXB 2) was the major product synthesised by all three species of shark studied ( Chil. griseum, Carcharhinus plumbeus, Carch. melanopterus), with 6-keto PGF 1 α a minor product. Other fish studied showed a varied pattern of prostanoid synthesis. The synthesis of these prostanoids was almost completely blocked by preincubation of the whole blood cells from catfish and shark with indomethacin (0.5 μM) suggesting the involvement of cyclooxygenase-mediated prostanoid synthesis.