Measurement of oxidatively induced base lesions in liver from Wistar rats of different ages

Mitochondrial and nuclear DNA were isolated from the livers of young (6–7 month) and old (23–24 month) Wistar rats and the levels of 10 different oxidatively induced lesions were analyzed by gas chromatography/mass spectrometry. This is the first study to measure several different oxidatively induce...

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Bibliographic Details
Published in:Free radical biology & medicine 1999-08, Vol.27 (3), p.456-462
Main Authors: Anson, R.Michael, Sentürker, Sema, Dizdaroglu, Miral, Bohr, Vilhelm A
Format: Article
Language:English
Subjects:
Aging
Aging - metabolism
Animals
DNA Damage
DNA, Mitochondrial - genetics
Free Radicals
Gas Chromatography-Mass Spectrometry
GC/MS
Hydrolysis
In Vitro Techniques
Liver - metabolism
Male
Mitochondrial DNA
Nucleotides - metabolism
Oxidation-Reduction
Oxidative DNA damage
Rats
Rats, Wistar
Species Specificity
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Summary:Mitochondrial and nuclear DNA were isolated from the livers of young (6–7 month) and old (23–24 month) Wistar rats and the levels of 10 different oxidatively induced lesions were analyzed by gas chromatography/mass spectrometry. This is the first study to measure several different oxidatively induced base lesions in both mitochondrial and nuclear DNA as a function of age. No significant age effects were observed for any lesion. Furthermore, contrary to expectations, we did not observe elevated levels of oxidatively induced base lesions in mitochondrial DNA. This contrasts with 50-fold differences reported for several lesions between mitochondrial and nuclear DNA from porcine liver (Zastawny et al., Free Radic. Biol. Med. 24:722–725, 1998). The fact that different lesion levels are observed even when similar techniques are employed emphasizes that the role of oxidative mitochondrial DNA damage and its repair in aging must continue to be the subject of intense investigation. Questions concerning endogenous levels of damage should be revisited as existing methods are improved and new methods become available.
ISSN:0891-5849
1873-4596
DOI:10.1016/S0891-5849(99)00091-X