Direct Analysis and Identification of Helicobacter and Campylobacter Species by MALDI-TOF Mass Spectrometry

• Published in: Analytical chemistry (Washington) 1999-08, Vol.71 (16), p.3416-3419
• Main Authors: Winkler, Martin A, Uher, John, Cepa, Steven
• Format: Article
• Language: English
• Subjects: Animals; Bacteria; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Campylobacter - isolation & purification; Chemistry; Databases, Factual; Fundamental and applied biological sciences. Psychology; Helicobacter - isolation & purification; Microbiology; Scientific imaging; Sheep; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac990135r

Campylobacter jejuni, Campylobacter fetus, and Campylobacter coli were compared with Helicobacter pylori and Helicobacter mustelae by direct analysis of individual cultured colonies in 50% methanol−water with a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS). H. pylori and Campylobacter species from blood agar culture produced unique, complex spectra with over 25 different ions in mass/charge (m/z) range from 2 000 to 62 000. A biomarker for H. pylori was centered around m/z 58 268, and H. mustelae was distinguished from H. pylori by its ions at m/z 49 608 and 57 231. Campylobacters could be distinguished from Helicobacters by their lack of ions around m/z 58 000 and 61 000 as well as distinguishing biomarkers of lower m/z:  10 074 and 25 478 for C. coli; m/z 10 285 and 12 901 for C. jejuni; m/z 10 726 and 11 289 for C. fetus. MALDI-TOF MS is a rapid and direct method for detection of these potentially pathogenic bacteria from culture.