Quantification of osteopontin in the urine of healthy and stone-forming men

Osteopontin (OPN) is one of the most important components in calcium stone matrix, but its role in stone formation is not clear. Since quantitative data regarding the excretion of OPN are necessary to assess its role, we have developed a quantitative enzyme-linked immunosorbent assay (ELISA) for OPN...

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Published in:Urolithiasis 1999-08, Vol.27 (4), p.225-230
Main Authors: Yasui, T, Fujita, K, Hayashi, Y, Ueda, K, Kon, S, Maeda, M, Uede, T, Kohri, K
Format: Article
Language:English
Subjects:
Adult
Aged
Calcium - urine
Creatinine - urine
Enzyme-Linked Immunosorbent Assay
Humans
Kidney stones
Linear Models
Male
Men
Middle Aged
Osteopontin
Recurrence
Reference Values
Sialoglycoproteins - urine
Urinary Calculi - urine
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container_end_page 230
container_issue 4
container_start_page 225
container_title Urolithiasis
container_volume 27
creator Yasui, T
Fujita, K
Hayashi, Y
Ueda, K
Kon, S
Maeda, M
Uede, T
Kohri, K
description Osteopontin (OPN) is one of the most important components in calcium stone matrix, but its role in stone formation is not clear. Since quantitative data regarding the excretion of OPN are necessary to assess its role, we have developed a quantitative enzyme-linked immunosorbent assay (ELISA) for OPN, and measured the urinary OPN concentrations in urolithiasis patients. Forty-seven men with urinary stones composed chiefly of calcium oxalate participated in the study. The controls were 13 normal healthy male volunteers. Urine samples were collected early in the morning and analyzed by a quantitative ELISA employing purified polyclonal antibodies to synthesized OPN aminopolypeptides. The urinary ratio of the concentrations of OPN and creatinine (OPN/Cre) in the urolithiasis patients (0.039 +/- 0.029) was significantly lower than that in the control subjects (0.062 +/- 0.030) (P
doi_str_mv 10.1007/s002400050114
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Since quantitative data regarding the excretion of OPN are necessary to assess its role, we have developed a quantitative enzyme-linked immunosorbent assay (ELISA) for OPN, and measured the urinary OPN concentrations in urolithiasis patients. Forty-seven men with urinary stones composed chiefly of calcium oxalate participated in the study. The controls were 13 normal healthy male volunteers. Urine samples were collected early in the morning and analyzed by a quantitative ELISA employing purified polyclonal antibodies to synthesized OPN aminopolypeptides. The urinary ratio of the concentrations of OPN and creatinine (OPN/Cre) in the urolithiasis patients (0.039 +/- 0.029) was significantly lower than that in the control subjects (0.062 +/- 0.030) (P&lt;0.05). Single stone formers (n = 26; 0.050 +/- 0.020) had significantly higher OPN/Cre ratios compared with recurrent stone formers (n = 21; 0.031 +/- 0.021) (P&lt;0. 05). 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source Springer Nature
subjects Adult
Aged
Calcium - urine
Creatinine - urine
Enzyme-Linked Immunosorbent Assay
Humans
Kidney stones
Linear Models
Male
Men
Middle Aged
Osteopontin
Recurrence
Reference Values
Sialoglycoproteins - urine
Urinary Calculi - urine
title Quantification of osteopontin in the urine of healthy and stone-forming men
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