novel integral membrane protein is differentially expressed in the chick growth plate and maps to chromosome 1

The growth plate is a specialised region of cartilage located at the growing ends of long bones in higher vertebrates. It is responsible for longitudinal bone growth and is under the control of many local and systemic factors. The growth plate consists of an orderly arrangement of small proliferativ...

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Bibliographic Details
Published in:Animal genetics 1999-08, Vol.30 (4), p.300-303
Main Authors: Lester, D. H., Farquharson, C., Paton, 1I. R., Seawright, E., Jefferies, D., Whitehead, C. C., Burt, D. W., Houston, B.
Format: Article
Language:English
Subjects:
5' Untranslated Regions
agarose gel differential display
amino acid sequences
Animals
autosomal dwarfism
Base Sequence
Bone Development - genetics
cell division
cell membranes
chicken genome mapping
chickens
Chickens - genetics
Chickens - metabolism
chondrocyte
Chondrocytes - metabolism
Chromosome Mapping
complementary DNA
dileucine motif
DNA Primers - genetics
Female
genbank/aj131560
Gene Expression
gene location
genetic polymorphism
growth plate
Growth Plate - cytology
Growth Plate - metabolism
loci
Male
membrane proteins
Membrane Proteins - genetics
Membrane Proteins - metabolism
messenger RNA
molecular sequence data
nucleotide sequences
open reading frames
Pedigree
Percoll gradient centrifugation
Polymorphism, Single-Stranded Conformational
RACE
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Summary:The growth plate is a specialised region of cartilage located at the growing ends of long bones in higher vertebrates. It is responsible for longitudinal bone growth and is under the control of many local and systemic factors. The growth plate consists of an orderly arrangement of small proliferative and larger mature hypertrophic chondrocytes. This paper describes the isolation by differential display of a 988-bp cDNA fragment derived from a transcript that is more highly expressed in proliferating rather than hypertrophic chondrocytes of the chick growth plate. Using 3' RACE, a further 939 bp of cDNA sequence was obtained. The 1.9 kb sequence contains a 924-bp open reading frame encoding an unknown 308 amino acid protein. This protein has a putative transmembrane domain near its N-terminus and three dileucine motifs at its carboxy tail. This gene was expressed in all other tissues examined. A polymorphism was identified by SSCP analysis and the gene was mapped to the centromeric region of the short arm of chicken chromosome 1, close to the locus for autosomal dwarfism.
ISSN:0268-9146
1365-2052
DOI:10.1046/j.1365-2052.1999.00473.x