Genetic organization of the lipopolysaccharide O-antigen biosynthetic locus of Leptospira borgpetersenii serovar Hardjobovis

Leptospiral LPS plays a critical role in immunity to leptospirosis and forms the basis for serological classification of Leptospira . However, neither the structure of leptospiral LPS nor the genetics of its biosynthesis have been elucidated. A probe derived from the rhamnose biosynthetic genes of L...

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Bibliographic Details
Published in:Microbial pathogenesis 1999-08, Vol.27 (2), p.105-117
Main Authors: Kalambaheti, Thareerat, Bulach, Dieter M., Rajakumar, Kumar, Adler, Ben
Format: Article
Language:English
Subjects:
Amino Acid Sequence
animal diseases
animal health
AO antigen
bacterial infections
Bacterial Proteins - genetics
Bacteriology
Biological and medical sciences
Chromosome Walking
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Genetics
glycosyltransferase
Glycosyltransferases - genetics
human health and safety
Leptospira - genetics
Leptospira - immunology
Leptospira borgpetersenii
medicine
Microbiology
Molecular Sequence Data
O Antigens - biosynthesis
Open Reading Frames - genetics
rfb gene
rfb locus, TDP-rhamnose, glycosyltransferases, Wzy-dependent LPS biosynthesis, Leptospira
rhamnose
Rhamnose - biosynthesis
Rhamnose - genetics
Sequence Alignment
Sequence Analysis, DNA
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Summary:Leptospiral LPS plays a critical role in immunity to leptospirosis and forms the basis for serological classification of Leptospira . However, neither the structure of leptospiral LPS nor the genetics of its biosynthesis have been elucidated. A probe derived from the rhamnose biosynthetic genes of L. interrogans serovar Copenhageni was used to identify the rfb locus of L. borgpetersenii serovar Hardjobovis. Chromosome walking and sequence analysis revealed an rfb locus spanning 36.7 kb, which consists of 31 ORFs transcribed in the same direction. Clusters of genes were identified which encode proteins related to enzymes involved in the biosynthesis of activated sugars including rhamnose. Additional ORFs in the locus encode glycosyltransferases for the assembly of the O-antigen subunit and integral membrane proteins for the transport of O-antigen subunits through the membrane and assembly into LPS.
ISSN:0882-4010
1096-1208
DOI:10.1006/mpat.1999.0285