Isolation and Characterization of a Novel Bladder Cancer Cell Line: Inhibition by Epidermal Growth Factor

A novel continuous cell line, designated BC3c, was established from a surgical biopsy of an invasive solid transitional cell carcinoma of the bladder derived from an 82-yr-old Caucasian female. BC3c cells were near-triploid bearing multiple structural and numerical chromosome anomalies. The epitheli...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 1998-10, Vol.34 (9), p.722-728
Main Authors: Pratsinis, H, Saetta, A, Gagos, S, Davaris, P
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Cancer
Carcinoma, Transitional Cell - genetics
Carcinoma, Transitional Cell - pathology
Cell culture techniques
Cell Division - drug effects
Cell growth
Cell lines
Codons
Cultured cells
Epidermal Growth Factor - pharmacology
Female
Genetic mutation
Growth, Differentiation, and Senescence
Humans
Karyotyping
Transitional cell carcinoma
Tumor cell line
Tumor Cells, Cultured
Urinary bladder
Urinary Bladder Neoplasms - genetics
Urinary Bladder Neoplasms - pathology
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Summary:A novel continuous cell line, designated BC3c, was established from a surgical biopsy of an invasive solid transitional cell carcinoma of the bladder derived from an 82-yr-old Caucasian female. BC3c cells were near-triploid bearing multiple structural and numerical chromosome anomalies. The epithelial origin of the cancer cells was indicated by the expression of cytokeratins 8 and 19 as well as by the absence of mesenchymal markers. Polymerase chain reaction-restriction-fragment length polymorphisms and single-strand conformation polymorphism mutation detection assays did not reveal any mutations in H-ras codon 12 and K-ras codons 12 and 13. In addition, no mutation in specific hot-spot codons of the p53 gene and no accumulation of the p53 protein were observed. BC3c cells grew rapidly in vitro, even in the absence of exogenous growth factors, because they were found to stimulate their growth in an autocrine manner. BC3c cells were found to express the epidermal growth factor-receptor (EGF-r) abundantly, but in contrast to other established bladder cancer cell lines, human recombinant epidermal growth factor inhibited the cells' proliferation in vitro. These features render the newly established bladder cancer cell line BC3c a useful tool for further experimentation.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-998-0068-z