Analysis of viral glycoproteins by glycosidic digestion inside a polyacrylamide gel

We adapted the method described by Cleveland et al. (1977); (Peptide mapping by limited proteolysis in sodium dodecyl sulphate and analysis by gel electrophoresis. J. Biol. Chem. 252, 1102–1106) to study the glycosidic residues linked to the viral glycoproteins of two enveloped viruses: Junin virus...

Full description

Saved in:
Bibliographic Details
Published in:Journal of virological methods 1999-07, Vol.80 (2), p.217-221
Main Authors: Grutadauria, Sergio, Castilla, Viviana, Zapata, Marta, Mersich, Susana
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cercopithecus aethiops
Electrophoresis, Polyacrylamide Gel - methods
Fundamental and applied biological sciences. Psychology
Glycoproteins - chemistry
Glycoside Hydrolases - metabolism
Glycosides - metabolism
Glycosidic residues
Iodine Radioisotopes - metabolism
Junin virus
Junin virus - chemistry
Junin virus - isolation & purification
Microbiology
Morphology, structure, chemical composition, physicochemical properties
Peptide Mapping - methods
Rubella virus
Rubella virus - chemistry
Rubella virus - isolation & purification
Vero Cells
Viral glycoproteins
Viral Proteins - chemistry
Virology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We adapted the method described by Cleveland et al. (1977); (Peptide mapping by limited proteolysis in sodium dodecyl sulphate and analysis by gel electrophoresis. J. Biol. Chem. 252, 1102–1106) to study the glycosidic residues linked to the viral glycoproteins of two enveloped viruses: Junin virus (JV) and rubella virus (RV). Radioiodinated glycoproteins were obtained from purified virions, isolated from SDS–polyacrylamide gels and then hydrolysed by specific glycosidases inside a second gel. N-linked oligosaccharides, mannose and galactose were found as terminal residues in the JV-G1 glycoprotein. Mannose and N-glycans of complex hybrid type were present on RV glycoproteins.
ISSN:0166-0934
1879-0984
DOI:10.1016/S0166-0934(99)00005-1