Deletion of the 389 N-terminal residues of the transcriptional activator AREA does not result in nitrogen metabolite derepression in Aspergillus nidulans

Utilizing a homologous gene replacement in order to retain the native promoter and 5' and 3' untranslated messenger regions (and thereby ensure physiological validity), we have shown that deletion of the N-terminal 389 amino acids of the transcriptional activator AREA does not result in ni...

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Bibliographic Details
Published in:Journal of Bacteriology 1998-11, Vol.180 (21), p.5762-5764
Main Authors: Caddick, M.X, Arst, H.N. Jr
Format: Article
Language:English
Subjects:
Aspergillus nidulans
Aspergillus nidulans - genetics
Aspergillus nidulans - metabolism
Aspergillus nidulellus
deletions
Fungal Proteins - genetics
Fungal Proteins - metabolism
Gene Deletion
gene expression
genes
genetic regulation
Genetics and Molecular Biology
mutants
nitrate reductase
Nitrogen - metabolism
regulator genes
Trans-Activators - genetics
transcription (genetics)
transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Utilizing a homologous gene replacement in order to retain the native promoter and 5' and 3' untranslated messenger regions (and thereby ensure physiological validity), we have shown that deletion of the N-terminal 389 amino acids of the transcriptional activator AREA does not result in nitrogen metabolite derepression in Aspergillus nidulans. Our results provide no evidence for a modulating interaction involving the N terminus of AREA and contrast with those of H.K. Lamb, A.L. Dodds, D.R. Swatman, E. Cairns, and A.R. Hawkins (J. Bacteriol. 179:6649-6656, 1997), who used nontargeted ectopic copies of a construct containing a heterologous promoter and untranslated regions. Results obtained with this deletion mutant, nevertheless, provide further evidence for the dispensability of large portions of AREA.
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.180.21.5762-5764.1998