Calcium dependency of arachidonic acid incorporation into cellular phospholipids of different cell types

Ca 2+-independent phospholipase A 2 (iPLA 2) is involved in the incorporation of arachidonic acid (AA) into resting macrophages by the generation of the lysophospholipid acceptor. The role of iPLA 2 in AA remodeling in different cells was evaluated by studying the Ca 2+ dependency of AA uptake from...

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Bibliographic Details
Published in:Prostaglandins & other lipid mediators 1999-07, Vol.57 (5), p.341-350
Main Authors: Daniele, José J, Fidelio, Gerardo D, Bianco, Ismael D
Format: Article
Language:English
Subjects:
Animals
Arachidonic acid
Arachidonic Acid - metabolism
Calcium - metabolism
Chick Embryo
Chick glial cells
Culture Media
Human neutrophils
Human platelets
Humans
In Vitro Techniques
Neutrophils - metabolism
Phospholipid remodeling
Phospholipids - metabolism
Rat fibroblasts
Rats
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Summary:Ca 2+-independent phospholipase A 2 (iPLA 2) is involved in the incorporation of arachidonic acid (AA) into resting macrophages by the generation of the lysophospholipid acceptor. The role of iPLA 2 in AA remodeling in different cells was evaluated by studying the Ca 2+ dependency of AA uptake from the medium, the incorporation into cellular phospholipids, and the effect of the iPLA 2 inhibitor bromoenol lactone on these events. Uptake and esterification of AA into phospholipids were not affected by Ca 2+ depletion in human polymorphonuclear neutrophils and rat fibroblasts. The uptake was Ca 2+ independent in chick embryo glial cells, but the incorporation into phospholipids was partially dependent on extracellular Ca 2+. Both events were fully dependent on extra and intracellular Ca 2+ in human platelets. In human polymorphonuclear neutrophils, the kinetics of incorporation in several isospecies of phospholipids was not affected by the absence of Ca 2+ at short times (
ISSN:1098-8823
DOI:10.1016/S0090-6980(98)00084-7