Densitometric thin layer chromatographic analysis of tretinoin and erythromycin in lotions for topical use in acne treatment

A TLC-method was developed to analyse tretinoin and erythromycin in a lotion in the presence of several excipients. Erythromycin was separated on a silica gel plate and a mobile phase with dichloromethane, methanol and ammonia 25% (60:6:1 (v/v/v)), tretinoin on a C 18 RP plate with acetonitrile and...

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Bibliographic Details
Published in:European journal of pharmaceutics and biopharmaceutics 1999-07, Vol.48 (1), p.53-58
Main Authors: Gabriëls, Margriet, Brisaert, Myriam, Plaizier-Vercammen, Jacqueline
Format: Article
Language:English
Subjects:
Acne
Acne Vulgaris - drug therapy
Administration, Topical
Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - analysis
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
Calibration
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Color
Densitometry
Erythromycin
Erythromycin - administration & dosage
Erythromycin - analysis
Excipients
General pharmacology
Indicators and Reagents
Keratolytic Agents - administration & dosage
Keratolytic Agents - analysis
Medical sciences
Ointments
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Thin-layer chromatography
Topical treatment
Tretinoin
Tretinoin - administration & dosage
Tretinoin - analysis
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Summary:A TLC-method was developed to analyse tretinoin and erythromycin in a lotion in the presence of several excipients. Erythromycin was separated on a silica gel plate and a mobile phase with dichloromethane, methanol and ammonia 25% (60:6:1 (v/v/v)), tretinoin on a C 18 RP plate with acetonitrile and water (50:25 (v/v)) as mobile phase, adding 1 ml acetic acid for the separation of the excipients and erythromycin. The derivatization for both was done with a dipping reagent, consisting of anisaldehyde, sulphuric acid and acetic acid (respectively 1, 2 and 10% (v/v/v)) and dissolved in chloroform/alcohol 94% v/v (60:30 (v/v)) for erythromycin and alcohol 94%/water (50:40 (v/v)) for tretinoin. These TLC-systems were quantitatively evaluated in terms of stability of the colour, precision, accuracy and calibration, proving the utility in the analysis of the lotion.
ISSN:0939-6411
1873-3441
DOI:10.1016/S0939-6411(99)00021-1