Proteinase 3 mRNA expression is induced in monocytes but not in neutrophils of patients with cystic fibrosis

Proteinase 3 (PR3), a serine proteinase which can degrade lung tissue, is present in the cystic fibrosis (CF) sputum. In the present study, PR3 protein and mRNA expression was determined in circulating neutrophils and monocytes. CF neutrophils contained similar PR3 concentrations as healthy controls...

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Bibliographic Details
Published in:FEBS letters 1999-09, Vol.457 (3), p.437-440
Main Authors: Just, Jocelyne, Moog-Lutz, Christel, Houzel-Charavel, Anne, Canteloup, Sandrine, Grimfeld, Alain, Witko-Sarsat, Véronique, Cayre, Yvon E.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
Bacterial Infections - complications
Bacterial Infections - drug therapy
Bacterial Infections - enzymology
CF, cystic fibrosis
Child
Child, Preschool
Cystic fibrosis
Cystic Fibrosis - complications
Cystic Fibrosis - drug therapy
Cystic Fibrosis - enzymology
Female
Humans
Infant
Inflammation
Lung disease
Male
Monocytes - enzymology
MPO, myeloperoxidase
Myeloblastin
NE, neutrophil elastase
Neutrophils - enzymology
Peroxidase - genetics
PR3, proteinase 3
Proteinase 3
Reference Values
RNA, Messenger - analysis
RNA, Messenger - drug effects
RT-PCR, reverse transcription-polymerase chain reaction
Serine Endopeptidases - drug effects
Serine Endopeptidases - genetics
Serine Endopeptidases - metabolism
Serine proteinase
SLPI, secretory leucoprotease inhibitor
Sputum - chemistry
Up-Regulation
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Summary:Proteinase 3 (PR3), a serine proteinase which can degrade lung tissue, is present in the cystic fibrosis (CF) sputum. In the present study, PR3 protein and mRNA expression was determined in circulating neutrophils and monocytes. CF neutrophils contained similar PR3 concentrations as healthy controls and poorly expressed PR3 mRNA. In contrast, CF monocytes showed significantly higher PR3 concentrations than controls, together with an upregulation of PR3 mRNA expression especially during pulmonary exacerbation. Interestingly, antibiotic treatment fully abrogated PR3 mRNA expression and decreased PR3 protein in monocytes. Our findings highlight a potential role of monocyte-derived PR3 in CF-associated airway inflammation.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(99)01098-4