Primary cultured human thymic epithelial cells express both membrane-bound and soluble HLA-G translated products

This report demonstrates that both membrane-bound and soluble HLA-G isoforms are present in primary cultured human thymic epithelial cells (TEC). HLA-G transcriptional isoforms have been detected by RT-PCR, using different sets of HLA-G specific primers. A flow cytometry analysis, using two anti-HLA...

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Bibliographic Details
Published in:Journal of reproductive immunology 1999-07, Vol.43 (2), p.225-234
Main Authors: Mallet, Valérie, Fournel, Sylvie, Schmitt, Christian, Campan, Agnès, Lenfant, Françoise, Le Bouteiller, Philippe
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Membrane - metabolism
Cells, Cultured
Epithelial Cells - cytology
Fundamental and applied biological sciences. Psychology
Gene expression
histocompatibility antigen HLA
Histocompatibility Antigens Class I - biosynthesis
Histocompatibility Antigens Class I - genetics
HLA Antigens - biosynthesis
HLA Antigens - genetics
HLA-G
HLA-G Antigens
Humans
Isoforms
Molecular and cellular biology
Molecular genetics
Protein Biosynthesis
Solubility
Thymic epithelial cells
Thymus Gland - cytology
Thymus Gland - immunology
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Summary:This report demonstrates that both membrane-bound and soluble HLA-G isoforms are present in primary cultured human thymic epithelial cells (TEC). HLA-G transcriptional isoforms have been detected by RT-PCR, using different sets of HLA-G specific primers. A flow cytometry analysis, using two anti-HLA-G mAbs, namely 87G and BFL.1, revealed the presence of HLA-G translated products at the cell surface of a subpopulation of TEC. Finally, it was shown that HLA-G soluble forms were secreted in TEC culture supernatant, using a sandwich elisa with BFL.1 and W6/32 mAbs. These results confirm and extent those previously described showing that HLA-G expressing cells were detectable by immunohistochemistry in thymic medullary epithelial cells.
ISSN:0165-0378
1872-7603
DOI:10.1016/S0165-0378(99)00036-4