Iron-Binding Catechols Oxidating Lignin and Chlorolignin

• Published in: Biochemical and biophysical research communications 1998-10, Vol.251 (2), p.399-402
• Main Authors: Parra, Carolina, Rodriguez, Jaime, Baeza, Jaime, Freer, Juanita, Durán, Nelson
• Format: Article
• Language: English
• Subjects: 3,4-Dihydroxyphenylacetic Acid - chemistry; catechol; chlorolignin; Dioxanes; Hydroxybenzoates - chemistry; Iron - chemistry; Iron Chelating Agents - chemistry; Kinetics; lignin; Lignin - analogs & derivatives; Lignin - chemistry; Oxidation-Reduction; siderophore; Spectrometry, Fluorescence; Spectrophotometry
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1998.9315

Iron-chelating low-molecular-weight compounds or catecholate siderophores have been suggested to be involved in wood biodegradation. To help in understanding the mechanism involved in the enzyme-like activity of catecholate siderophores, the oxidative properties of 2,3-dihydroxybenzoic acid (DHBA) and 3,4-dihydroxyphenylacetic acid (DHPAA) chelated with iron were studied. The pH and catechol/Fe(III) ratios were optimized foro-dianisidine oxidation, obtaining a maximum at pH 7.0, in the absence of buffer, and a catechol/Fe(III) ratio of 1:2 to DHBA and 1:1 to DHPAA was found. Under these conditions, the catechols were able to reduce Fe(III) to Fe(II) acting like siderophore models. The Fe(III) complex of DHBA and of DHPAA degraded dioxane-lignin in 60% after 2 h and 85% after 24 h, respectively. DHBA/Fe(III) oxidized the bleaching effluent (E1) in 80% in 5 min under the studied conditions.