Molecular Epidemiology of Rabies in Thailand

For the purpose of making clear the dynamics of rabies viruses that are prevalent among dogs in Asia, especially Thailand, nucleoprotein (N) genes of isolates derived from Thailand were partially sequenced, and a phylogenetic analysis was performed on the basis of the sequencing data. Firstly, all 2...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1999, Vol.43(6), pp.551-559
Main Authors: Ito, Naoto, Sugiyama, Makoto, Oraveerakul, Kanisak, Piyaviriyakul, Prapruddee, Lumlertdacha, Boonlert, Arai, Yoko T., Tamura, Yutaka, Mori, Yoshio, Minamoto, Nobuyuki
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
China - epidemiology
Dog Diseases - epidemiology
Dog Diseases - virology
Dogs
Epidemiology
Epidemiology. Vaccinations
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
General aspects
Genes, Viral
Infectious diseases
Japan - epidemiology
Medical sciences
Microbiology
Molecular Epidemiology
Molecular Sequence Data
Nucleoprotein gene
Nucleoproteins - genetics
Phylogeny
Polymorphism, Restriction Fragment Length
Rabies - epidemiology
Rabies - veterinary
Rabies - virology
Rabies virus
Rabies virus - classification
Rabies virus - genetics
Rabies virus - isolation & purification
Reverse Transcriptase Polymerase Chain Reaction
RFLP analysis
Sequence Analysis, DNA
Thailand
Thailand - epidemiology
Viral Proteins - genetics
Virology
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Summary:For the purpose of making clear the dynamics of rabies viruses that are prevalent among dogs in Asia, especially Thailand, nucleoprotein (N) genes of isolates derived from Thailand were partially sequenced, and a phylogenetic analysis was performed on the basis of the sequencing data. Firstly, all 27 isolates from Thailand belonged to one group that was distantly related to an isolate from China and was separated into at least six lineages. On the other hand, the isolate from Japan was related to viruses from the Arctic. Secondly, in order to analyze the diversity of the N gene more conveniently, restriction fragment length polymorphism (RFLP) analysis was performed on the N gene of 27 isolates from Thailand. The RFLP analysis could distinguish the lineages of each isolate, and the lineages of additional 34 isolates were deduced by this method. On examination of the geographical distribution of the six lineages, based on the results of phylogenetic and RFLP analyses, it was clear that infection cycles of the rabies virus in Thailand have tended to be maintained endemically.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1999.tb02441.x