Activation of mononuclear cells by interleukin-12: An in vivo study in chimpanzees

Interleukin (IL)-12 is considered a central regulator of host resistance against a variety of pathogens. Therefore, IL-12 has been advocated as a potential therapeutic agent in infections. To determine the in vivo effects of IL-12 on mononuclear cells involved in the host immune response, four chimp...

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Bibliographic Details
Published in:Journal of clinical immunology 1999-07, Vol.19 (4), p.231-238
Main Authors: LAUW, F. N, TE VELDE, A. A, DEKKERS, P. E. P, SPEELMAN, P, AERTS, J. M. F. G, HACK, C. E, VAN DEVENTER, S. J. H, VAN DER POLL, T
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Biological and medical sciences
Biomarkers
Cytotoxicity, Immunologic
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Granzymes
Hexosaminidases - blood
Immunobiology
Interleukin-12 - pharmacology
Killer Cells, Natural
Leukocyte Count
Leukocytes, Mononuclear - drug effects
Lymphocyte Count
Lymphokines, interleukins ( function, expression)
Monocytes - cytology
Pan troglodytes - immunology
Phagocytes
Regulatory factors and their cellular receptors
Serine Endopeptidases - blood
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Summary:Interleukin (IL)-12 is considered a central regulator of host resistance against a variety of pathogens. Therefore, IL-12 has been advocated as a potential therapeutic agent in infections. To determine the in vivo effects of IL-12 on mononuclear cells involved in the host immune response, four chimpanzees received an intravenous injection of recombinant IL-12 (1 microgram/kg). IL-12 induced a sustained decrease in lymphocyte counts, with decreases in CD3+/CD4+ and CD3+/CD8+ cells, while monocyte counts showed a transient increase. IL-12 injection resulted in a shift toward a Th1-mediated immune response as indicated by increased interferon-gamma production during whole-blood stimulation, while not influencing IL-4 production. IL-12-induced activation of NK cells and phagocytes, as indicated by increased NK cell cytotoxicity and increased plasma levels of granzymes A and B and of chitotriosidase activity. These data support the hypothesis that IL-12 may serve as a useful therapeutic agent in infections where a cell-mediated response is protective.
ISSN:0271-9142
1573-2592
DOI:10.1023/A:1020520130792