Integration of chicken cytogenetic and genetic maps: 18 new polymorphic markers isolated from BAC and PAC clones

As an approach to integrate the chicken genetic and cytogenetic maps, bacterial artificial chromosome (BAC) and P1‐derived artificial chromosome (PAC) clones were localized by fluorescence in situ hybridization (FISH) on chromosomes and by genetic mapping on the East Lansing and Compton reference fa...

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Bibliographic Details
Published in:Animal genetics 1998-10, Vol.29 (5), p.348-355
Main Authors: Morisson, M., Pitel, F., Fillon, V., Pouzadoux, A., Bergé, R., Vit, J. P., Zoorob, R., Auffray, C., Gellin, J., Vignal, A.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
C8A
Chickens - genetics
Chromosome Mapping
Classical genetics, quantitative genetics, hybrids
Cloning, Molecular
Cytogenetics
DNA Primers - genetics
Fundamental and applied biological sciences. Psychology
Genetic Linkage
Genetic Markers
Genetics of eukaryotes. Biological and molecular evolution
Genomic Library
In Situ Hybridization, Fluorescence
mapping
Microsatellite Repeats
polymorphism
Polymorphism, Genetic
Polymorphism, Single-Stranded Conformational
swine
Vertebrata
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Summary:As an approach to integrate the chicken genetic and cytogenetic maps, bacterial artificial chromosome (BAC) and P1‐derived artificial chromosome (PAC) clones were localized by fluorescence in situ hybridization (FISH) on chromosomes and by genetic mapping on the East Lansing and Compton reference families. Some of the clones used in this study were previously selected for the presence of potentially polymorphic (CA)n repeats and a microsatellite marker was developed when possible for genetic mapping. For other clones, a single strand conformational polymorphism (SSCP) was developed and used for this purpose. Between the two approaches, 18 markers linking the cytogenetic and genetic maps, seven on macrochromosomes and 11 on microchromosomes, were generated. Our results enabled the assignment and orientation of a linkage group to chromosome 3, together with the assignment of linkage groups to eight different microchromosomes, a fraction of the genome lacking mapping data and for which the degree of coverage by the genetic map was not well estimated previously.
ISSN:0268-9146
1365-2052
DOI:10.1046/j.1365-2052.1998.295348.x