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gene required for the regulation of photosynthetic light harvesting in the cyanobacterium Synechocystis 6803
A gene required for the short‐term regulation of photosynthetic light harvesting (the state transition) has been identified in the cyanobacterium Synechocystis sp. PCC6803. The open reading frame is designated sll1926 in the complete Synechocystis gene sequence. The deduced amino acid sequence has n...
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| Published in: | Molecular microbiology 1999-09, Vol.33 (5), p.1050-1058 |
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| creator | Emlyn-Jones, D Ashby, M.K Mullineaux, C.W |
| description | A gene required for the short‐term regulation of photosynthetic light harvesting (the state transition) has been identified in the cyanobacterium Synechocystis sp. PCC6803. The open reading frame is designated sll1926 in the complete Synechocystis gene sequence. The deduced amino acid sequence has no homologues in current sequence databases and no recognizable sequence motifs. It encodes a putative integral membrane protein of 16 kDa, which we have designated RpaC (regulator of phycobilisome association C). Fluorescence measurements of an insertional inactivation mutant of rpaC (Δsll1926) show that it is specifically unable to perform state transitions. Δsll1926 has approximately wild‐type levels of PS1, PS2 and phycobilisomes. Measurements of oxygen evolution and uptake show Δsll1926 to have no deficiency in electron transport rates. In vitro [γ‐32P]‐ATP labelling experiments suggest that RpaC is not the 15 kDa membrane phosphoprotein previously implicated in state transitions. Δsll1926 grows more slowly than the wild type only at very low light intensities. |
| doi_str_mv | 10.1046/j.1365-2958.1999.01547.x |
| format | article |
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PCC6803. The open reading frame is designated sll1926 in the complete Synechocystis gene sequence. The deduced amino acid sequence has no homologues in current sequence databases and no recognizable sequence motifs. It encodes a putative integral membrane protein of 16 kDa, which we have designated RpaC (regulator of phycobilisome association C). Fluorescence measurements of an insertional inactivation mutant of rpaC (Δsll1926) show that it is specifically unable to perform state transitions. Δsll1926 has approximately wild‐type levels of PS1, PS2 and phycobilisomes. Measurements of oxygen evolution and uptake show Δsll1926 to have no deficiency in electron transport rates. In vitro [γ‐32P]‐ATP labelling experiments suggest that RpaC is not the 15 kDa membrane phosphoprotein previously implicated in state transitions. Δsll1926 grows more slowly than the wild type only at very low light intensities.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1046/j.1365-2958.1999.01547.x</identifier><identifier>PMID: 10476038</identifier><language>eng</language><publisher>Oxford BSL: Blackwell Science Ltd</publisher><subject>Amino Acid Sequence ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Cell Division - genetics ; Cell Membrane - chemistry ; Cyanobacteria - genetics ; Fluorescence ; Freshwater ; Gene Expression Regulation, Bacterial ; Light ; light harvesting complex ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Molecular Sequence Data ; Mutation ; Open Reading Frames ; Oxygen - metabolism ; Phosphorylation ; photosynthesis ; Photosynthetic Reaction Center Complex Proteins - genetics ; Phycobilisomes ; Pigments, Biological - metabolism ; Signal Transduction ; state transition ; Synechocystis ; Temperature ; Time Factors</subject><ispartof>Molecular microbiology, 1999-09, Vol.33 (5), p.1050-1058</ispartof><rights>Copyright Blackwell Scientific Publications Ltd. Sep 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5637-d0f2c323de85e3a90c95eb45572398e92491fdddc0e95769b49770086c421e2e3</citedby><cites>FETCH-LOGICAL-c5637-d0f2c323de85e3a90c95eb45572398e92491fdddc0e95769b49770086c421e2e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10476038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Emlyn-Jones, D</creatorcontrib><creatorcontrib>Ashby, M.K</creatorcontrib><creatorcontrib>Mullineaux, C.W</creatorcontrib><title>gene required for the regulation of photosynthetic light harvesting in the cyanobacterium Synechocystis 6803</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>A gene required for the short‐term regulation of photosynthetic light harvesting (the state transition) has been identified in the cyanobacterium Synechocystis sp. PCC6803. The open reading frame is designated sll1926 in the complete Synechocystis gene sequence. The deduced amino acid sequence has no homologues in current sequence databases and no recognizable sequence motifs. It encodes a putative integral membrane protein of 16 kDa, which we have designated RpaC (regulator of phycobilisome association C). Fluorescence measurements of an insertional inactivation mutant of rpaC (Δsll1926) show that it is specifically unable to perform state transitions. Δsll1926 has approximately wild‐type levels of PS1, PS2 and phycobilisomes. Measurements of oxygen evolution and uptake show Δsll1926 to have no deficiency in electron transport rates. In vitro [γ‐32P]‐ATP labelling experiments suggest that RpaC is not the 15 kDa membrane phosphoprotein previously implicated in state transitions. Δsll1926 grows more slowly than the wild type only at very low light intensities.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Cell Division - genetics</subject><subject>Cell Membrane - chemistry</subject><subject>Cyanobacteria - genetics</subject><subject>Fluorescence</subject><subject>Freshwater</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Light</subject><subject>light harvesting complex</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Open Reading Frames</subject><subject>Oxygen - metabolism</subject><subject>Phosphorylation</subject><subject>photosynthesis</subject><subject>Photosynthetic Reaction Center Complex Proteins - genetics</subject><subject>Phycobilisomes</subject><subject>Pigments, Biological - metabolism</subject><subject>Signal Transduction</subject><subject>state transition</subject><subject>Synechocystis</subject><subject>Temperature</subject><subject>Time Factors</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqNkk9v1DAQxS0EotuFrwAWB24J_hM78YEDqoBWatVDW4mb5XUmiVfZeGsnpfn2OE2FEBc42fL83tPMPCOEKckpKeSnfU65FBlTosqpUionVBRl_vgCbX4XXqINUYJkvGI_TtBpjHtCKCeSv0YnyaSUhFcb1LcwAA5wP7kANW58wGO3PLRTb0bnB-wbfOz86OM8pMroLO5d2424M-EB4uiGFrvhSWRnM_idsSMENx3wzTyA7bydExSxrAh_g141po_w9vncortvX2_PzrPL6-8XZ18uMyskL7OaNMxyxmuoBHCjiFUCdoUQJeOqAsUKRZu6ri0BJUqpdoUqS0IqaQtGgQHfoo-r7zH4-yk1qQ8uWuh7M4Cfok4wowUX_wRpydP6Uitb9OEvcO-nMKQhNFVSUCGLMkHVCtngYwzQ6GNwBxNmTYlectN7vcSjl3j0kpt-yk0_Jum7Z_9pd4D6D-EaVAI-r8BP18P838b66upiuSX9-1XfGK9NG1zUdzds-RBMUUZYwX8Basuv9w</recordid><startdate>199909</startdate><enddate>199909</enddate><creator>Emlyn-Jones, D</creator><creator>Ashby, M.K</creator><creator>Mullineaux, C.W</creator><general>Blackwell Science Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>199909</creationdate><title>gene required for the regulation of photosynthetic light harvesting in the cyanobacterium Synechocystis 6803</title><author>Emlyn-Jones, D ; Ashby, M.K ; Mullineaux, C.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5637-d0f2c323de85e3a90c95eb45572398e92491fdddc0e95769b49770086c421e2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Cell Division - genetics</topic><topic>Cell Membrane - chemistry</topic><topic>Cyanobacteria - genetics</topic><topic>Fluorescence</topic><topic>Freshwater</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Light</topic><topic>light harvesting complex</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Open Reading Frames</topic><topic>Oxygen - metabolism</topic><topic>Phosphorylation</topic><topic>photosynthesis</topic><topic>Photosynthetic Reaction Center Complex Proteins - genetics</topic><topic>Phycobilisomes</topic><topic>Pigments, Biological - metabolism</topic><topic>Signal Transduction</topic><topic>state transition</topic><topic>Synechocystis</topic><topic>Temperature</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Emlyn-Jones, D</creatorcontrib><creatorcontrib>Ashby, M.K</creatorcontrib><creatorcontrib>Mullineaux, C.W</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Emlyn-Jones, D</au><au>Ashby, M.K</au><au>Mullineaux, C.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>gene required for the regulation of photosynthetic light harvesting in the cyanobacterium Synechocystis 6803</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>1999-09</date><risdate>1999</risdate><volume>33</volume><issue>5</issue><spage>1050</spage><epage>1058</epage><pages>1050-1058</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>A gene required for the short‐term regulation of photosynthetic light harvesting (the state transition) has been identified in the cyanobacterium Synechocystis sp. PCC6803. The open reading frame is designated sll1926 in the complete Synechocystis gene sequence. The deduced amino acid sequence has no homologues in current sequence databases and no recognizable sequence motifs. It encodes a putative integral membrane protein of 16 kDa, which we have designated RpaC (regulator of phycobilisome association C). Fluorescence measurements of an insertional inactivation mutant of rpaC (Δsll1926) show that it is specifically unable to perform state transitions. Δsll1926 has approximately wild‐type levels of PS1, PS2 and phycobilisomes. Measurements of oxygen evolution and uptake show Δsll1926 to have no deficiency in electron transport rates. In vitro [γ‐32P]‐ATP labelling experiments suggest that RpaC is not the 15 kDa membrane phosphoprotein previously implicated in state transitions. Δsll1926 grows more slowly than the wild type only at very low light intensities.</abstract><cop>Oxford BSL</cop><pub>Blackwell Science Ltd</pub><pmid>10476038</pmid><doi>10.1046/j.1365-2958.1999.01547.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular microbiology, 1999-09, Vol.33 (5), p.1050-1058 |
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| language | eng |
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| source | Wiley |
| subjects | Amino Acid Sequence Bacterial Proteins - genetics Bacterial Proteins - metabolism Cell Division - genetics Cell Membrane - chemistry Cyanobacteria - genetics Fluorescence Freshwater Gene Expression Regulation, Bacterial Light light harvesting complex Membrane Proteins - genetics Membrane Proteins - metabolism Molecular Sequence Data Mutation Open Reading Frames Oxygen - metabolism Phosphorylation photosynthesis Photosynthetic Reaction Center Complex Proteins - genetics Phycobilisomes Pigments, Biological - metabolism Signal Transduction state transition Synechocystis Temperature Time Factors |
| title | gene required for the regulation of photosynthetic light harvesting in the cyanobacterium Synechocystis 6803 |
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