A Perifusion System to Control Oxygen Concentration in Cell Suspensions

A system is described for the perifusion of cells with a perifusate containing oxygen at concentrations defined by the user. The metabolic competency of cells in this system was assessed by measuring total ATP turnover for human platelets. Human platelets, which are small (radius of 1–2 μm) anucleat...

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Bibliographic Details
Published in:Analytical biochemistry 1998-10, Vol.263 (2), p.208-213
Main Authors: Arthur, P.G., Ngo, C.T., Wakeford, C.M.
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Blood Platelets - cytology
Blood Platelets - metabolism
Cells, Cultured
Humans
Oxygen - metabolism
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Description
Summary:A system is described for the perifusion of cells with a perifusate containing oxygen at concentrations defined by the user. The metabolic competency of cells in this system was assessed by measuring total ATP turnover for human platelets. Human platelets, which are small (radius of 1–2 μm) anucleate cells involved in blood clotting, maintained ATP turnover over a 4-h period at 37°C. Oxygen concentration in this system was controlled by adjusting the proportions of air-saturated and nitrogen-saturated perifusates. Examples of oxygen consumption and lactate output as a function of oxygen concentration are described.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1998.2787