Sevoflurane mimics ischemic Preconditioning effects on coronary flow and nitric oxide release in isolated hearts

Like ischemic preconditioning, certain volatile anesthetics have been shown to reduce the magnitude of ischemia/ reperfusion injury via activation of K+ adenosine triphosphate (ATP)-sensitive (K(ATP)) channels. The purpose of this study was (1) to determine if ischemic preconditioning (IPC) and sevo...

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Bibliographic Details
Published in:Anesthesiology (Philadelphia) 1999-09, Vol.91 (3), p.701-712
Main Authors: NOVALIJA, E, FUJITA, S, KAMPINE, J. P, STOWE, D. F
Format: Article
Language:English
Subjects:
Anesthetics, Inhalation - pharmacology
Anesthetics. Neuromuscular blocking agents
Animals
Biological and medical sciences
Coronary Circulation - drug effects
Guinea Pigs
Heart - drug effects
Hemodynamics - drug effects
In Vitro Techniques
Ischemic Preconditioning
Medical sciences
Methyl Ethers - pharmacology
Neuropharmacology
Nitric Oxide - biosynthesis
Pharmacology. Drug treatments
Potassium Channels - drug effects
Sevoflurane
Superoxides - metabolism
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Summary:Like ischemic preconditioning, certain volatile anesthetics have been shown to reduce the magnitude of ischemia/ reperfusion injury via activation of K+ adenosine triphosphate (ATP)-sensitive (K(ATP)) channels. The purpose of this study was (1) to determine if ischemic preconditioning (IPC) and sevoflurane preconditioning (SPC) increase nitric oxide release and improve coronary vascular function, as well as mechanical and electrical function, if given for only brief intervals before global ischemia of isolated hearts; and (2) to determine if K(ATP) channel antagonism by glibenclamide (GLB) blunts the cardioprotective effects of IPC and SPC. Guinea pig hearts were isolated and perfused with Krebs-Ringer's solution at 55 mm Hg and randomly assigned to one of seven groups: (1) two 2-min total coronary occlusions (preconditioning, IPC) interspersed with 5 min of normal perfusion; (2) two 2-min occlusions interspersed with 5 min of perfusion while perfusing with GLB (IPC+GLB); (3) SPC (3.5%) for two 2-min periods; (4) SPC+GLB for two 2-min periods; (5) no treatment before ischemia (control [CON]); (6) CON+GLB; and (7) no ischemia (time control). Six minutes after ending IPC or SPC, hearts of ischemic groups were subjected to 30 min of global ischemia and 75 min of reperfusion. Left-ventricular pressure, coronary flow, and effluent NO concentration ([NO]) were measured. Flow and NO responses to bradykinin, and nitroprusside were tested 20-30 min before ischemia or drug treatment and 30-40 min after reperfusion. After ischemia, compared with before (percentage change), left-ventricular pressure and coronary flow, respectively, recovered to a greater extent (P
ISSN:0003-3022
1528-1175
DOI:10.1097/00000542-199909000-00023