Physical mapping and partial genetic characterization of the Lactobacillus delbrueckii subsp. bulgaricus bacteriophage lb539

A restriction map was constructed of the 37 kb genome of the temperate Lactobacillus delbrueckii subsp. bulgaricus bacteriophage lb539. Restriction analysis and Southern hybridization experiments detected variable levels of homologous regions among the genomes of lb539 and the L. delbrueckii referen...

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Bibliographic Details
Published in:Archives of virology 1999-01, Vol.144 (8), p.1503-1512
Main Authors: Auad, L, Räisänen, L, Raya, R. R, Alatossava, T
Format: Article
Language:English
Subjects:
bacteriophages
Bacteriophages - genetics
Biological and medical sciences
DNA
DNA, Viral - analysis
evolution
Fundamental and applied biological sciences. Psychology
genes
Genetics
Lactobacillus - virology
Lactobacillus delbrueckii bulgaricus
Lactobacillus delbrueckii subsp. bulgaricus
Microbiology
Phage lb539
Restriction Mapping
Southern blotting
structural proteins
Viral Structural Proteins - analysis
Virology
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Description
Summary:A restriction map was constructed of the 37 kb genome of the temperate Lactobacillus delbrueckii subsp. bulgaricus bacteriophage lb539. Restriction analysis and Southern hybridization experiments detected variable levels of homologous regions among the genomes of lb539 and the L. delbrueckii reference phages LL-H (virulent) and mv4 (temperate). The principal homology was observed at the regions encoding the structural proteins. These studies allowed us to construct a partial genetic map of phage lb539 for lysin, the main structural tail protein and the packaging region genes. Furthermore, a short 1.5 kb DNA fragment of the prolate-headed JCL1032 phage genome was observed to be highly homologous with the DNA of the isometric-headed lb539, mv4 and LL-H phages. The described distribution of the homologous regions between the genomes of the phages lb539, LL-H, mv4 and JCL1032 presented here supports the modular evolution theory of the bacteriophages.
ISSN:0304-8608
1432-8798
DOI:10.1007/s007050050606