Effect of surgical stress on benzodiazepine receptors as a consequence of placebo pellet implantation in rat: An autoradiographic study

This paper reports modifications in benzodiazepine (BZ) receptors induced by minimally invasive surgery, such as pellet implantation, a widely used surgical process for chronic drug administration. The intrinsic stress induced by this manipulation on BZ receptors was analysed in an autoradiographic...

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Bibliographic Details
Published in:Brain research bulletin 1999-08, Vol.49 (6), p.413-418
Main Authors: Revilla, Victoria, Soria, Carlos, Aller, Maria Isabel, Calvo, Pedro, Fernández- López, Arsenio
Format: Article
Language:English
Subjects:
Animals
Autoradiography
Autoradiography - methods
Benzodiazepine
Biological and medical sciences
Brain - metabolism
BZ1
BZ2
Central nervous system
Central neurotransmission. Neuromudulation. Pathways and receptors
Drug Implants
Flunitrazepam - pharmacokinetics
Fundamental and applied biological sciences. Psychology
GABA
Male
Minimally Invasive Surgical Procedures
Organ Specificity
Placebos
Rats
Rats, Wistar
Receptors, GABA-A - metabolism
Stress, Physiological - metabolism
Surgical stress
Tritium
Vertebrates: nervous system and sense organs
zolpidem
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Summary:This paper reports modifications in benzodiazepine (BZ) receptors induced by minimally invasive surgery, such as pellet implantation, a widely used surgical process for chronic drug administration. The intrinsic stress induced by this manipulation on BZ receptors was analysed in an autoradiographic saturation study determining the affinity (KD) and total number (Bmax) of binding sites of a number of brain areas from the mesencephalon, cerebral cortex, hippocampus, and cerebellum. The radioligand used for the study was [3H]flunitrazepam, which permitted overall characterization of BZ binding sites. Use of the specific BZ1 agonist zolpidem as an inhibitor of this radioligand permitted the direct characterization of subtype 2 (BZ2) and the indirect characterization of subtype 1 receptor (BZ1). Significant increases in Bmax and KD values were observed in pellet-implanted animals with respect to those not implanted. The results support the notion of an up-regulation of these receptors, mainly in BZ1 receptors, following sustained desensitization as a result of the surgical stress induced by pellet implantation.
ISSN:0361-9230
1873-2747
DOI:10.1016/S0361-9230(99)00073-8