The Two-Exon Gene of the Human Forkhead Transcription Factor FREAC-2 (FKHL6) Is Located at 6p25.3

• Published in: Genomics (San Diego, Calif.) Calif.), 1998-11, Vol.53 (3), p.387-390
• Main Authors: Blixt, Åsa, Mahlapuu, Margit, Bjursell, Cecilia, Darnfors, Catarina, Johannesson, Tonnie, Enerbäck, Sven, Carlsson, Peter
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Base Sequence; Binding Sites - genetics; Biological and medical sciences; Chromosome Mapping; Chromosomes, Human, Pair 6 - genetics; Consensus Sequence; CpG Islands; DNA Primers - genetics; DNA, Complementary - genetics; DNA-Binding Proteins - genetics; Exons; Forkhead Transcription Factors; Fundamental and applied biological sciences. Psychology; Genes. Genome; Humans; Hybrid Cells; In Situ Hybridization, Fluorescence; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Polymerase Chain Reaction; Trans-Activators - genetics
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1998.5451

The gene for the human transcription factor forkhead related activator 2 (FREAC-2; HGMW-approved symbol FKHL6) has been characterized and found to consist of two exons separated by an intron of 3.6 kb. The first exon encodes the forkhead DNA-binding domain and one of the transcriptional activation domains, AD2. The second exon contains the coding sequence corresponding to the C-terminal activation domain AD1. The full-length FREAC-2 protein is predicted to be 444 amino acids, which adds 39 amino acids to the previously published partial cDNA sequence. A 2-kb CG island is centered around the 5′ end of theFREAC-2gene. Fluorescencein situhybridization was used to localize the humanFREAC-2gene to chromosomal position 6p24–p25, and the localization was further refined by radiation hybrid mapping to 6p25.3.