Analysis of a translational enhancer present within the 5'-terminal sequence of the genomic RNA of potato virus S

• Published in: Archives of virology 1999, Vol.144 (7), p.1451-1461
• Main Authors: Turner, R.L, Glynn, M, Taylor, S.C, Cheung, M.K, Spurr, C, Twell, D, Foster, G.D
• Format: Article
• Language: English
• Subjects: 5' untranslated regions; 5' Untranslated Regions - chemistry; 5' Untranslated Regions - metabolism; Animals; Base Sequence; Biological and medical sciences; Carlavirus - genetics; Enhancer Elements, Genetic; enhancer sequences; enzyme activity; Fundamental and applied biological sciences. Psychology; gene expression; Genetics; leaves; luciferase; Microbiology; Molecular Sequence Data; open reading frames; Phytopathology. Animal pests. Plant and forest protection; Plant viruses and viroids; Potato virus S; Protein Biosynthesis; protoplasts; Rabbits; RNA, Viral - chemistry; RNA, Viral - metabolism; Solanum tuberosum - virology; Systematics. Structure, properties and multiplication. Genetics; tobacco; transgenic plants; translation (genetics); Virology; wheat germ
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s007050050601

When present as a transcript leader the 5′ untranslated sequence from the potato virus S genomic RNA molecule enhances translation of a downstream open reading frame both in vitro and in vivo. Translational enhancement was 30-fold in rabbit reticulocyte lysate and 15 fold in wheat germ above translation from a transcript with a synthetic leader. Transient expression experiments using tobacco protoplasts and particle bombardment of leaf tissue resulted in enhancement of fourteen and five-fold, respectively, above translation with a synthetic leader. In stably transformed plants the PVS 5′UTR enhanced translation yield ca. 5-fold compared with a synthetic 5′UTR.