One type of chalcone synthase gene expressed during embryogenesis regulates the flavonoid accumulation in citrus cell cultures

To elucidate the relationship between the expression of chalcone synthase (CHS) genes and the production of flavonoid in citrus cell cultures, two cDNA clones encoding CHS were isolated (CitCHS1 and CitCHS2) from the citrus. The accumulation of CitCHS2 mRNA was notably induced by embryogenesis but C...

Full description

Saved in:
Bibliographic Details
Published in:Plant and cell physiology 1999-06, Vol.40 (6), p.651-655
Main Authors: Moriguchi, T. (kaj-ot), Kita, M, Tomono, Y, Endo Inagaki, T, Omura, M
Format: Article
Language:English
Subjects:
Acyltransferases - genetics
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
CALCONA
CELL CULTURE
Cells, Cultured
CHALCONE
Chalcone synthase
CHALCONES
CITRUS
Citrus - cytology
Citrus - enzymology
Citrus - genetics
CULTIVO DE CELULAS
CULTURE DE CELLULE
DESARROLLO EMBRIONARIO
DEVELOPPEMENT EMBRYONNAIRE
EMBRYONIC DEVELOPMENT
Flavonoid biosynthesis
FLAVONOIDE
FLAVONOIDES
FLAVONOIDS
Flavonoids - metabolism
GENE
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
GENES
Isoenzymes - genetics
Molecular Sequence Data
Plant Leaves
RNA, Messenger - genetics
Seeds
Somatic embryogenesis
Transcription, Genetic
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To elucidate the relationship between the expression of chalcone synthase (CHS) genes and the production of flavonoid in citrus cell cultures, two cDNA clones encoding CHS were isolated (CitCHS1 and CitCHS2) from the citrus. The accumulation of CitCHS2 mRNA was notably induced by embryogenesis but CitCHS1 mRNA was not. There was no detectable accumulation of flavonoid in the undifferentiated calli, but flavonoid accumulated after the morphological changes to embryoids. These results indicate that two CHS genes differentially expressed during citrus somatic embryogenesis and CitCHS2 may regulate the accumulation of flavonoid in citrus cell cultures.
ISSN:0032-0781
1471-9053
DOI:10.1093/oxfordjournals.pcp.a029589