The broad host range plasmid pLF1311 from Lactobacillus fermentum VKM1311

Abstract The complete nucleotide sequence (2389 bp) of the cryptic plasmid pLF1311 from Lactobacillus fermentum VKM1311 was determined. DNA sequence analysis revealed the putative coding regions for a replicative protein (RepB), its repressor (RepA) and double-stranded (dso) and single-stranded (sso...

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Bibliographic Details
Published in:FEMS microbiology letters 1999-09, Vol.178 (1), p.47-53
Main Authors: Aleshin, Vladimir V., Semenova, Elena V., Doroshenko, Vera G., Jomantas, Yurgis V., Tarakanov, Boris V., Livshits, Vitaliy A.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteria
Bacterial Proteins
Coliforms
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA Helicases
DNA Replication
DNA, Single-Stranded - chemistry
DNA-Binding Proteins
E coli
Escherichia coli
Gram-positive bacteria
Host range
Lactobacillus - genetics
Lactobacillus fermentum
Lactobacillus plasmid
Maintenance
Microbiology
Mobilizable vector
Molecular Sequence Data
Nucleotide sequence
Nucleotides
Phosphoprotein Phosphatases - genetics
Plasmids
Plasmids - genetics
Protein Structure, Secondary
Proteins - genetics
Replication
Rolling circle replication
Sequence Alignment
Staphylococcus aureus
Trans-Activators
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Summary:Abstract The complete nucleotide sequence (2389 bp) of the cryptic plasmid pLF1311 from Lactobacillus fermentum VKM1311 was determined. DNA sequence analysis revealed the putative coding regions for a replicative protein (RepB), its repressor (RepA) and double-stranded (dso) and single-stranded (sso) origins. pLF1311 belongs to the pE194 family of rolling circle-replicating plasmids. A derivative of pLF1311 that contains the cat gene of plasmid pC194 of Staphylococcus aureus and the oriT of RP4 was constructed and transferred by conjugative mobilization from Escherichia coli to various Gram-positive bacteria. The stable maintenance of this derivative was shown in some strains of Lactobacillus, Lactococcus, Enterococcus and Bacillus under non-selective conditions.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1999.tb13758.x