Molecular diagnosis of group B coltiviruses infections

The group-B of genus Coltivirus encompasses isolates from humans, ticks or mosquitoes collected in Indonesia and China. Subgroup-B1 includes the strain JKT/dsR-7075 and subgroup-B2 strains JKT/dsR-6423, JKT/dsR-6969, JKT/dsR–7043 and the Banna virus. Data are described for the PCR-based diagnosis of...

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Bibliographic Details
Published in:Journal of virological methods 1999-08, Vol.81 (1), p.39-45
Main Authors: Billoir, Frédérique, Attoui, Houssam, Simon, Sophie, Gallian, Pierre, de Micco, Philippe, de Lamballerie, Xavier
Format: Article
Language:English
Subjects:
Animals
Anopheles - virology
Arboviruses RT–PCR
Banna virus
Base Sequence
Biological and medical sciences
Brain - virology
Cells, Cultured
Coltivirus
Coltivirus - genetics
Coltivirus - isolation & purification
Culex - virology
Epidemiology
Female
Fundamental and applied biological sciences. Psychology
Group B coltiviruses
Human viral diseases
Indonesian coltiviruses
Infectious diseases
Medical sciences
Mice
Mice, Inbred BALB C
Microbiology
Miscellaneous
Molecular Sequence Data
Reoviridae Infections - diagnosis
Reoviridae Infections - virology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - blood
RNA, Viral - isolation & purification
Sensitivity and Specificity
Tropical medicine
Viral diseases
Virology
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Description
Summary:The group-B of genus Coltivirus encompasses isolates from humans, ticks or mosquitoes collected in Indonesia and China. Subgroup-B1 includes the strain JKT/dsR-7075 and subgroup-B2 strains JKT/dsR-6423, JKT/dsR-6969, JKT/dsR–7043 and the Banna virus. Data are described for the PCR-based diagnosis of infection by group B coltiviruses. Sets of primers were designed from the sequences of the 7th, 9th and 12th viral segments and RT–PCR assays were developed. Consensus primers permitted the detection of all known isolates of subgroup 1 or 2. Viral strains could be characterised further using primers specific for type B2a or B2b, or based on the length of the amplification products. All primers gave negative results when using RNAs from Orbiviruses or Group-A coltiviruses. These primers permitted the detection of Group-B coltiviruses-RNA in infected mouse blood at the acute stage of the disease. Accordingly, they could be used for the diagnosis of infection in humans.
ISSN:0166-0934
1879-0984
DOI:10.1016/S0166-0934(99)00055-5