Parapoxviruses: potential alternative vectors for directing the immune response in permissive and non-permissive hosts

Parapoxvirus (PPV) represents a genus of the poxviridae, and particularly PPV ovis (Orf virus, OV) seems to offer several potential advantages for the use of vector vaccine. Therefore, we started to investigate the genome of the highly attenuated OV strain D1701, which was only poorly characterised...

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Bibliographic Details
Published in:Journal of biotechnology 1999-08, Vol.73 (2), p.235-242
Main Authors: Rziha, Hanns-Joachim, Henkel, Marco, Cottone, Rosita, Meyer, Marlene, Dehio, Christoph, Büttner, Mathias
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biotechnology
Chromosome Mapping
deletions
Endothelial Growth Factors - genetics
Fundamental and applied biological sciences. Psychology
Gene Deletion
Genes
Genetic engineering
Genetic Vectors
genome
Genome, Viral
Genomic map
Growth kinetics
Health. Pharmaceutical industry
Humans
Immunology
Industrial applications and implications. Economical aspects
Lymphokines - genetics
Orf virus
Orf virus - genetics
Orf virus - immunology
Orf virus - pathogenicity
Orf virus strain D1701
Parapoxvirus
Parapoxvirus - genetics
Parapoxvirus - immunology
Poxvirus
Production of active biomolecules
vaccine development
Vaccines
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - genetics
Vaccins
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
Vector
VEGF deletion mutant
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Summary:Parapoxvirus (PPV) represents a genus of the poxviridae, and particularly PPV ovis (Orf virus, OV) seems to offer several potential advantages for the use of vector vaccine. Therefore, we started to investigate the genome of the highly attenuated OV strain D1701, which was only poorly characterised until now. Due to recombination of non-homologous sequences, part of the right hand end of the D1701 genome was duplicated and translocated to the opposite end of the genome. As a consequence gene deletion had occurred and the inverted terminal repeat region is increased. Results are described to identify viral genes, which are non-essential for virus replication and potentially influence viral pathogenesis, virulence, and host immunity. In more detail, we analysed the expression and functional activity of the OV-specific vascular endothelial growth factor (VEGF) gene homologue. Finally the construction and production of a D1701 mutant lacking the VEGF gene homologue is reported.
ISSN:0168-1656
1873-4863
DOI:10.1016/S0168-1656(99)00141-8