Characterization of unusual G8 rotavirus strains isolated from Egyptian children

We report the first detection of P[14], G8 rotaviruses isolated in Egypt from the stool of children participating in a 3 year study of rotavirus epidemiology. Two strains, EGY1850 and EGY2295, were characterized by a serotyping enzyme immunoassay (EIA), virus neutralization, and sequence analysis of...

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Bibliographic Details
Published in:Archives of virology 1999-01, Vol.144 (7), p.1381-1396
Main Authors: Holmes, J. L, Kirkwood, C. D, Gerna, G, Clemens, J. D, Rao, M. R, Naficy, A. B, Abu-Elyazeed, R, Savarino, S. J, Glass, R. I, Gentsch, J. R
Format: Article
Language:English
Subjects:
Animals
Antigens, Viral
Biological and medical sciences
Capsid - genetics
Capsid Proteins
Cattle
Child, Preschool
children
Egypt
enzyme immunoassays
genes
Genotype
geographical distribution
Human viral diseases
Humans
Infant
Infant, Newborn
Infectious diseases
Medical sciences
monoclonal antibodies
neutralization
neutralization tests
Rotavirus - genetics
Rotavirus - immunology
Rotavirus - isolation & purification
sequence analysis
serotypes
Serotyping
Viral diseases
Viral diseases of the digestive system
viruses
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Summary:We report the first detection of P[14], G8 rotaviruses isolated in Egypt from the stool of children participating in a 3 year study of rotavirus epidemiology. Two strains, EGY1850 and EGY2295, were characterized by a serotyping enzyme immunoassay (EIA), virus neutralization, and sequence analysis of the genes encoding VP7 and the VP8* portion of the VP4 gene. These two strains shared a high level of homology of their VP7s (87.8% nucleotide [nt], 97.2% amino acid [aa]) and VP4s (89.6% nt, 97.1% aa) and had the highest VP7 identity to serotype G8 (>82% nt, >92% aa) and VP4 identity to genotype P[14] (≥81% nt, >91% aa) strains. Serological results with a VP7 G8-specific and VP4 P[14]-specific neutralizing monoclonal antibodies supported the genetic classification of EGY1850 and EGY2295 as P[14], G8. Genogroup analysis supports earlier findings that human G8 rotaviruses may be genetically related to bovine rotaviruses. These findings demonstrate that our understanding of the geographic distribution of rotavirus strains is incomplete, emphasize the need to monitor rota- virus serotypes, and extend the known distribution of serotype G8 and genotype P[14] strains in Africa.
ISSN:0304-8608
1432-8798
DOI:10.1007/s007050050594