Detection of nitrosyl–iron complexes by proton-electron–double-resonance imaging

The nitrogen monoxide radical (NO ) forms paramagnetic mono- and dinitrosyl–iron complexes in biologic tissues. To establish a noninvasive technique for in vivo NO imaging, we evaluated the suitability of these complexes as magnetic resonance (MR) contrast agents, making use of the ability of the un...

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Bibliographic Details
Published in:Free radical biology & medicine 1999-09, Vol.27 (5), p.636-646
Main Authors: Mülsch, Alexander, Lurie, David J, Seimenis, Ioannis, Fichtlscherer, Birgit, Foster, Margaret A
Format: Article
Language:English
Subjects:
Animals
Contrast Media - chemistry
Cysteine - analogs & derivatives
Cysteine - chemistry
Electron spin resonance
Electron Spin Resonance Spectroscopy
Free radicals
Iron - analysis
Liver - chemistry
Magnetic Resonance Imaging - methods
Male
Nitrogen monoxide
Nitrogen Oxides - analysis
Nitroprusside - chemistry
Nitrosyl–iron complex
Proton-electron–double-resonance imaging
Rat liver
Rats
Rats, Sprague-Dawley
Serum Albumin - chemistry
Sodium nitroprusside
Temperature
Time Factors
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Summary:The nitrogen monoxide radical (NO ) forms paramagnetic mono- and dinitrosyl–iron complexes in biologic tissues. To establish a noninvasive technique for in vivo NO imaging, we evaluated the suitability of these complexes as magnetic resonance (MR) contrast agents, making use of the ability of the unpaired electrons of the complexes to enter into dynamic nuclear polarization with water protons and hence produce enhancement on images generated by the technique of proton-electron–double-resonance imaging (PEDRI). Phantom solutions of synthetic nitrosyl–iron complexes (NICs) altered the signal intensity of PEDRI images. The dinitrosyl–iron complex (DNIC) with serum albumin induced a significantly larger signal alteration than the mononitrosyl–iron complex (MNIC) with dithiocarbamate. Exposure of rat liver to sodium nitroprusside (SNP) by ex vivo and in situ perfusion induced a composite X-band electron spin resonance (ESR) spectrum of the isolated liver characteristic of a MNIC and DNIC. On storage of the tissue, the MNIC signal disappeared and the DNIC signal intensity increased. Correspondingly, in cross-sectional PEDRI images taken at room temperature, the SNP-exposed livers initially exhibited a weak signal that strongly increased with time. In conclusion, NICs can be detected using PEDRI and could be exploited for in vivo NO imaging.
ISSN:0891-5849
1873-4596
DOI:10.1016/S0891-5849(99)00122-7